Detailed information of TA system TA196401

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 532360..532998
• Replicon: chromosome
• Accession: NZ_CP071441
• Organism: Escherichia coli strain EcPF20

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: S1F9G9
• Locus tag: J1F34_RS02615
• Protein ID: WP_000813794.1
• Coordinates: 532360..532536 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J1F34_RS02620
• Protein ID: WP_001270286.1
• Coordinates: 532582..532998 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F34_RS02595	527979..529193	-	1215	WP_001349372.1	BenE family transporter YdcO	-
J1F34_RS02600	529246..529782	+	537	WP_000429141.1	DNA-binding transcriptional regulator SutR	-
J1F34_RS02605	529855..531816	+	1962	WP_001307191.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J1F34_RS02610	531908..532138	-	231	WP_000494244.1	YncJ family protein	-
J1F34_RS02615	532360..532536	+	177	WP_000813794.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J1F34_RS02620	532582..532998	+	417	WP_001270286.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J1F34_RS02625	533077..534498	+	1422	WP_207268781.1	aminotransferase class I/II-fold pyridoxal phosphate-dependent enzyme	-
J1F34_RS02630	534532..535229	+	698	Protein_520	IS1 family transposase	-
J1F34_RS02635	535245..535898	+	654	Protein_521	ABC transporter ATP-binding protein	-
J1F34_RS02640	535899..536840	+	942	WP_001251319.1	ABC transporter permease	-
J1F34_RS02645	536830..537624	+	795	WP_000555481.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	526674..527939	1265

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (91-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6749.83 Da
• Isoelectric Point: 11.2298

>T196401 WP_000813794.1 NZ_CP071441:532360-532536 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T196401 NZ_CP095116:c2202973-2202764 [Staphylococcus aureus]
ATGATACTTCAAAATACGATTTACACAGCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAATATTAATATAATAGATGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTGTATTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15246.63 Da
• Isoelectric Point: 4.7386

>AT196401 WP_001270286.1 NZ_CP071441:532582-532998 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT196401 NZ_CP095116:c2203278-2202985 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATAAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Toxin

• PDB: 6HPB