Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 82510..83035 Replicon plasmid p1
Accession NZ_CP071440
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name ccdB Uniprot ID V0SSI5
Locus tag J1F19_RS24165 Protein ID WP_001159868.1
Coordinates 82510..82815 (-) Length 102 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID S1PPD8
Locus tag J1F19_RS24170 Protein ID WP_000813634.1
Coordinates 82817..83035 (-) Length 73 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS24150 78420..79586 - 1167 WP_000772446.1 plasmid-partitioning protein SopA -
J1F19_RS24155 80174..80929 - 756 WP_000852146.1 replication initiation protein RepE -
J1F19_RS24160 81703..82509 - 807 WP_000016982.1 site-specific integrase -
J1F19_RS24165 82510..82815 - 306 WP_001159868.1 type II toxin-antitoxin system toxin CcdB Toxin
J1F19_RS24170 82817..83035 - 219 WP_000813634.1 type II toxin-antitoxin system antitoxin CcdA Antitoxin
J1F19_RS24175 83743..84738 + 996 WP_000246635.1 hypothetical protein -
J1F19_RS24180 84742..85674 + 933 WP_000991831.1 hypothetical protein -
J1F19_RS24185 85967..86152 + 186 WP_001586223.1 hypothetical protein -
J1F19_RS24190 86153..87055 + 903 WP_024187432.1 hypothetical protein -
J1F19_RS24195 87057..87926 + 870 WP_001586225.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / mph(A) / sul1 / qacE / aadA5 / tet(B) / sul2 / aph(3'')-Ib / aph(6)-Id - 1..98697 98697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-100)

Antitoxin

Post-segregation antitoxin CcdA

(2-72)


Sequences

Toxin        


Download         Length: 102 a.a.        Molecular weight: 11706.51 Da        Isoelectric Point: 6.4674

>T196394 WP_001159868.1 NZ_CP071440:c82815-82510 [Escherichia coli]
MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEV
ADLSHRENDIKNAINLMFWGI

Download         Length: 306 bp

>T196394 NZ_CP095116:938662-938814 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin


Download         Length: 73 a.a.        Molecular weight: 8372.34 Da        Isoelectric Point: 4.7405

>AT196394 WP_000813634.1 NZ_CP071440:c83035-82817 [Escherichia coli]
MKQRITVTVDSDSYQLLKAYDVNISGLVSTTMQNEARRLRAERWKAENQEGMAEVARFIEMNGSFADENRDW

Download         Length: 219 bp

>AT196394 NZ_CP095116:938038-938637 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCTGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 4VUB
PDB 2VUB
PDB 7EPI
PDB 7EPG
PDB 3VUB
PDB 1X75
PDB 1VUB
PDB 7EPJ
PDB 3HPW
PDB 3G7Z

Antitoxin

Source ID Structure
PDB 3TCJ
PDB 2H3A
PDB 2ADN
PDB 2ADL
PDB 3HPW
PDB 2H3C
PDB 3G7Z
AlphaFold DB A0A829CQY2

References