Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) relBE/RHH(antitoxin)
Location 5251..5810 Replicon plasmid p1
Accession NZ_CP071440
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name relE Uniprot ID V0SVC8
Locus tag J1F19_RS23700 Protein ID WP_000421272.1
Coordinates 5251..5526 (-) Length 92 a.a.

Antitoxin (Protein)


Gene name relB Uniprot ID V0SRR3
Locus tag J1F19_RS23705 Protein ID WP_001387467.1
Coordinates 5526..5810 (-) Length 95 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS23670 1301..2041 - 741 WP_001586233.1 tyrosine-type recombinase/integrase -
J1F19_RS23675 2162..2344 - 183 WP_042004909.1 hypothetical protein -
J1F19_RS23680 2687..3027 - 341 Protein_3 type II toxin-antitoxin system ParD family antitoxin -
J1F19_RS23685 3487..4656 + 1170 WP_001072355.1 hypothetical protein -
J1F19_RS23690 4689..4817 + 129 Protein_5 IS481 family transposase -
J1F19_RS23695 4852..5145 - 294 WP_001105060.1 hypothetical protein -
J1F19_RS23700 5251..5526 - 276 WP_000421272.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J1F19_RS23705 5526..5810 - 285 WP_001387467.1 ribbon-helix-helix domain-containing protein Antitoxin
J1F19_RS23710 6415..7167 + 753 WP_000562172.1 IclR family transcriptional regulator -
J1F19_RS23715 7213..8178 - 966 WP_001022265.1 2-keto-3-deoxygluconate transporter -
J1F19_RS23720 8211..8591 - 381 WP_000710783.1 Rid family detoxifying hydrolase -
J1F19_RS23725 8616..9506 - 891 WP_001077068.1 dihydrodipicolinate synthase family protein -
J1F19_RS23730 9907..10347 + 441 Protein_13 zinc-binding dehydrogenase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid blaTEM-1B / mph(A) / sul1 / qacE / aadA5 / tet(B) / sul2 / aph(3'')-Ib / aph(6)-Id - 1..98697 98697


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(3-90)

Antitoxin

Ribbon-helix-helix protein, CopG

(14-51)


Sequences

Toxin        


Download         Length: 92 a.a.        Molecular weight: 10749.45 Da        Isoelectric Point: 10.0871

>T196393 WP_000421272.1 NZ_CP071440:c5526-5251 [Escherichia coli]
MELKWTSKALSDLSRLFDFLVLASKPAAARTVQSLTQAPVILLTHPRMGEQLFQFEPREVRRIFAGEYEIRYEINGQTIY
VLRLWHTRENR

Download         Length: 276 bp

>T196393 NZ_CP095116:424389-424709 [Staphylococcus aureus]
ATGAATTGGGAAATTAATGATTTGTTTAACGATTTGAAAGTGTTGAAAGATAGATTCGAAGATTTAAAGGATAATCATGG
TTGGCATTTTGATAATTTTTATCCTCATGAACCTAATCATGTTTTAAATAAAACCGAAGCGATTAAAGAAGGATTTTCTT
ATCATGAGAGACGTATTCACAATAATCAAATGTTTGATTTATTCCATCTCTATATAGAGCAGTTCGATAATATTATACAA
AAATTTCATGAAATAGAAAAAGCATCATCTGAGAACTTTGGCGAGGTATCAGATGACGCAAAGAATGTGAAAAAAGCATA
A

Antitoxin


Download         Length: 95 a.a.        Molecular weight: 10460.87 Da        Isoelectric Point: 5.6558

>AT196393 WP_001387467.1 NZ_CP071440:c5810-5526 [Escherichia coli]
MQMKNNAAQATKVITAHVPLPMADKVDQMAARLERSRGWVIKQALSAWLAQEEERNRLTLEALDDVTSGQVIDHQAVQAW
SDSLSTDNPLPVPR

Download         Length: 285 bp

>AT196393 NZ_CP095116:424177-424386 [Staphylococcus aureus]
ATGAATAATGAACAAAAAGAAGTGATAGAACACGTGGTTTATCAACTTGAGTTAAGTGTCATGAATAATTTGGAAAGTTA
TGAACACACTGAATATGTTAATGGTATTGAAGTGGTTTCAGAGGTTAGTCGTGAAAAGCACTTAGAATTGATAATGAAAT
GGTGCGCACAAGAATTAAAGAATAATTTTCAATTAGAGAAAGGAGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB V0SVC8

Antitoxin

Source ID Structure
AlphaFold DB A0A0E0Y880

References