Detailed information of TA system TA196387

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 4559511..4560216
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: J1F19_RS21325
• Protein ID: WP_000539521.1
• Coordinates: 4559830..4560216 (-)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J1F19_RS21320
• Protein ID: WP_001280945.1
• Coordinates: 4559511..4559840 (-)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS21305	4554668..4555579	+	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-
J1F19_RS21310	4555757..4558105	+	2349	WP_001305945.1	EAL domain-containing protein	-
J1F19_RS21315	4558113..4559441	+	1329	WP_001586846.1	GGDEF domain-containing protein	-
J1F19_RS21320	4559511..4559840	-	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
J1F19_RS21325	4559830..4560216	-	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J1F19_RS21330	4560442..4561767	-	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
J1F19_RS21335	4561980..4562363	+	384	WP_000497137.1	biofilm formation regulator BssR	-
J1F19_RS21340	4562474..4563589	+	1116	WP_207291673.1	PQQ-dependent sugar dehydrogenase	-
J1F19_RS21345	4563586..4564212	-	627	WP_001314584.1	glutathione S-transferase GstB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T196387 WP_000539521.1 NZ_CP071439:c4560216-4559830 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T196387 NZ_CP095112:c2004223-2004071 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACTGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT196387 WP_001280945.1 NZ_CP071439:c4559840-4559511 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT196387 NZ_CP095112:c2004847-2004248 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTAGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACTGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATATTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTGACTGGTGCTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTTGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
CGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: A0A829GCT6