Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4152842..4153460 Replicon chromosome
Accession NZ_CP071439
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J1F19_RS19525 Protein ID WP_001291435.1
Coordinates 4152842..4153060 (-) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J1F19_RS19530 Protein ID WP_000344800.1
Coordinates 4153086..4153460 (-) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS19490 4148131..4148703 + 573 WP_000779833.1 YbaY family lipoprotein -
J1F19_RS19495 4148734..4149045 - 312 WP_000409911.1 MGMT family protein -
J1F19_RS19505 4149424..4149777 + 354 WP_000878140.1 DUF1428 domain-containing protein -
J1F19_RS19510 4149819..4151369 - 1551 WP_001305493.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J1F19_RS19515 4151533..4152003 - 471 WP_000136192.1 YlaC family protein -
J1F19_RS19520 4152119..4152670 - 552 WP_000102564.1 maltose O-acetyltransferase -
J1F19_RS19525 4152842..4153060 - 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
J1F19_RS19530 4153086..4153460 - 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J1F19_RS19535 4154006..4157155 - 3150 WP_207291667.1 multidrug efflux RND transporter permease subunit AcrB -
J1F19_RS19540 4157178..4158371 - 1194 WP_001295833.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196386 WP_001291435.1 NZ_CP071439:c4153060-4152842 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T196386 NZ_CP095112:c903688-903500 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTCGGATTCGCTATTTTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT196386 WP_000344800.1 NZ_CP071439:c4153460-4153086 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT196386 NZ_CP095112:c903977-903717 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References