Detailed information of TA system TA196385

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): mosAT/AbiEii-AbiEi
• Location: 3954236..3955889
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: mosT
• Uniprot ID: A0A0X5EJM1
• Locus tag: J1F19_RS18615
• Protein ID: WP_001615045.1
• Coordinates: 3955005..3955889 (+)
• Length: 295 a.a.

Antitoxin (Protein)

• Gene name: mosA
• Uniprot ID: -
• Locus tag: J1F19_RS18610
• Protein ID: WP_001134467.1
• Coordinates: 3954236..3955018 (+)
• Length: 261 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS18585	3949689..3951014	-	1326	WP_001586702.1	DNA phosphorothioation-dependent restriction protein DptG	-
J1F19_RS18590	3951019..3952668	-	1650	WP_001586703.1	DNA phosphorothioation-dependent restriction protein DptF	-
J1F19_RS18595	3952945..3953169	-	225	WP_024187424.1	helix-turn-helix domain-containing protein	-
J1F19_RS18600	3953250..3953480	-	231	WP_001577779.1	helix-turn-helix domain-containing protein	-
J1F19_RS18605	3953549..3953859	+	311	Protein_3630	helix-turn-helix domain-containing protein	-
J1F19_RS18610	3954236..3955018	+	783	WP_001134467.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	Antitoxin
J1F19_RS18615	3955005..3955889	+	885	WP_001615045.1	nucleotidyl transferase AbiEii/AbiGii toxin family protein	Toxin
J1F19_RS18620	3956341..3957225	-	885	WP_001119037.1	LysR family transcriptional regulator	-
J1F19_RS18625	3957490..3958548	+	1059	WP_207291719.1	alpha/beta hydrolase	-
J1F19_RS18630	3958690..3959817	+	1128	WP_000171079.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Nucleotidyl transferase AbiEii toxin, Type IV TA system (14-209)

Antitoxin

• Transcriptional regulator AbiEi antitoxin, N-terminal domain (3-90)

Sequences

Toxin

Protein

• Length: 295 a.a.
• Molecular weight: 33151.42 Da
• Isoelectric Point: 7.7408

>T196385 WP_001615045.1 NZ_CP071439:3955005-3955889 [Escherichia coli]
MDLRDSYARQVKLLMAALPHVAKESCFALKGGTAINLFVQDFPRLSVDIDLAAINDALKRITASLNGRPGITAIRQENKA
DEKRIIVNTADAKIKIEVSPVWRGLLLPPAEMPVCERVEMEYGFTTMSVVSLADLYGGKICAALDRQHPRDLFDVLNMLE
KPGVMREIFDGFLCYLAGHPRPIAELLAPNWDTERITTLYAQEFSGMTQQETSLESLLSVTTLLPQALKSHLTDLDREFS
LSFKQNSPDWSRYRYPEIQHLPAIRWKQRNLAVLKDKNPAKYVAAVNKLERVLA

Nucleotide

• Length: 885 bp

>T196385 NZ_CP095109:c2582878-2582612 [Staphylococcus aureus]
ATGAGCAATTACACGGTTAAGATTAAAAATTCAGCGAAATCAGATTTAAAGAAAATAAAACATCCTTATTTAAAGAAGAT
ATTTATAGAAATTGTTGAGACTTTAAAAAATGATCCTTGTAAAATAATACAATCCTTTGAAAAATTAGAACCTAAATATT
TAGAGCGATATTCAAGAAGAATTAACCATCAGCACAGGGTCGTCTATACCATAGATGATCGAAATAAAGTAGTATTAATA
CTATCGGCATGGTCACATTATGATTAA

Antitoxin

Protein

• Length: 261 a.a.
• Molecular weight: 29093.39 Da
• Isoelectric Point: 8.4395

>AT196385 WP_001134467.1 NZ_CP071439:3954236-3955018 [Escherichia coli]
MPNYLNWLMQHTLPGQVLLQSWLTRNGIDRKLSYLYVKNGWLRRIAHGVYCRTGREPDWVDAVYCLQTQWEKPVRVAGLT
SLALQGHAHYTEPGKPQVWLALPAYVKLPGWFAAFEQSATFITLYTSGLTVDVTSLTTELKIGDRQLAGSAPELAAYEIA
CGVPSLISFEHADALFQGMNLLSPRKLQALLCASHSVKTNRVMLYLARRNGHPYFQYIDQSGIETGTGKRQIIPGGWLEP
DYQITVPRAFKPEGVEDGSA

Nucleotide

• Length: 783 bp

>AT196385 NZ_CP095109:c2583135-2582878 [Staphylococcus aureus]
ATGATTATCACTAGCCCTACAGAAGCGAGAAAAGATTTTTATCAATTACTAAAAAATGTTAATAATAATCACGAACCAAT
TTATATTAGTGGCAATAATGCCGAAAATAATGCTGTGATTATAGGTTTAGAAGATTGGAAAAGTATACAAGAGACAATAT
ATCTTGAATCTACTGGTACAATGGACAAAGTAAGAGAAAGAGAAAAAGATAGTAGTGGTACAACAAATATAGATGATATT
GATTGGGATAATCTTTAA

Structures

Toxin

• AlphaFold DB: A0A0X5EJM1