Detailed information of TA system TA196383

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 3672372..3672796
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J1F19_RS17315
• Protein ID: WP_001586653.1
• Coordinates: 3672608..3672796 (+)
• Length: 63 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: V0VEX8
• Locus tag: J1F19_RS17310
• Protein ID: WP_000125566.1
• Coordinates: 3672372..3672605 (+)
• Length: 78 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS17285	3667445..3667732	+	288	WP_000203747.1	ferredoxin-like protein FixX	-
J1F19_RS17290	3667791..3669122	+	1332	WP_001183198.1	MFS transporter	-
J1F19_RS17295	3669230..3669760	+	531	WP_000600725.1	glutathione-regulated potassium-efflux system oxidoreductase KefF	-
J1F19_RS17300	3669753..3671615	+	1863	WP_000377113.1	glutathione-regulated potassium-efflux system protein KefC	-
J1F19_RS17305	3671807..3672286	+	480	WP_000624375.1	type 3 dihydrofolate reductase	-
J1F19_RS17310	3672372..3672605	+	234	WP_000125566.1	type II toxin-antitoxin system CcdA family antitoxin	Antitoxin
J1F19_RS17315	3672608..3672796	+	189	WP_001586653.1	CcdB family protein	Toxin
J1F19_RS17320	3672793..3672909	+	117	WP_001389634.1	CcdB family protein	-
J1F19_RS17325	3672906..3673754	-	849	WP_000257195.1	bis(5'-nucleosyl)-tetraphosphatase (symmetrical) ApaH	-
J1F19_RS17330	3673761..3674138	-	378	WP_000610901.1	Co2+/Mg2+ efflux protein ApaG	-
J1F19_RS17335	3674141..3674962	-	822	WP_001065361.1	16S rRNA (adenine(1518)-N(6)/adenine(1519)-N(6))- dimethyltransferase RsmA	-
J1F19_RS17340	3674959..3675948	-	990	WP_000241280.1	4-hydroxythreonine-4-phosphate dehydrogenase PdxA	-
J1F19_RS17345	3675948..3677234	-	1287	WP_000800470.1	peptidylprolyl isomerase SurA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-57)

Antitoxin

• Post-segregation antitoxin CcdA (8-77)

Sequences

Toxin

Protein

• Length: 63 a.a.
• Molecular weight: 7029.22 Da
• Isoelectric Point: 8.7938

>T196383 WP_001586653.1 NZ_CP071439:3672608-3672796 [Escherichia coli]
MQFTVYRSRGSNAAFPFVIDVTSDIIGEINRSIVIPLTPIERFIRIRPPERLNTILLLVDGS

Nucleotide

• Length: 189 bp

>T196383 NZ_CP095109:c2201592-2201383 [Staphylococcus aureus]
ATGATACATCAAAATACGATTTACACATCGGGAATTGAAACAGAAGAACAAGTAAGTCAATTGACAGAACGCATTTCAAA
TATGATAGGTGTTCATCAAGTGAACATTAATATAATAGACGGTCAAGTAACTGTATCGTATGAGACACCAGCAAATTTGA
ATAGTATTGAAAAAGAAATCTATGATGAAGGATACAAAATTTTATTTTAG

Antitoxin

Protein

• Length: 78 a.a.
• Molecular weight: 8530.53 Da
• Isoelectric Point: 5.8062

>AT196383 WP_000125566.1 NZ_CP071439:3672372-3672605 [Escherichia coli]
MTAKRTTQSVTVTVDRELVNRARDAGLNMSATLTVALNAELKKHAATRWREENAEAIAALNQLADETGCFSDEYRSF

Nucleotide

• Length: 234 bp

>AT196383 NZ_CP095109:c2201897-2201604 [Staphylococcus aureus]
ATGACTGAACAAGATAATGCACATCATTCTGAACAAATAAAAACGAATCTTAAATCACGTTTAAATCGAATTGAAGGACA
AGTGAGAGCGATTAATCGCATGATTGAAGAAGATGTCTATTGTGATGATGTCCTTACGCAAATAAGAGCGACACGTTCGG
CGTTAAACAGTGTTGCGACAAAGTTATTAGAACAACATATGAAAAGTTGTATTATGAATAAAGTTAATCAAGGTGCTCAG
GAAGAGGCAATGGAAGAGTTATTAGTGACTTTTCAAAAATTGATTAAAGACTAA

Structures

Antitoxin

• AlphaFold DB: A0A7U9LTM9