Detailed information of TA system TA196381

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 3487238..3488070
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A7ZVJ9
• Locus tag: J1F19_RS16495
• Protein ID: WP_000854765.1
• Coordinates: 3487696..3488070 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: S1NM52
• Locus tag: J1F19_RS16490
• Protein ID: WP_001295723.1
• Coordinates: 3487238..3487606 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS16465	3484356..3484514	+	159	WP_001323397.1	DUF905 family protein	-
J1F19_RS16470	3484669..3485487	+	819	WP_001234716.1	DUF945 domain-containing protein	-
J1F19_RS16475	3485826..3486299	+	474	WP_001387789.1	antirestriction protein	-
J1F19_RS16480	3486315..3486791	+	477	WP_001186773.1	RadC family protein	-
J1F19_RS16485	3486854..3487075	+	222	WP_000692300.1	DUF987 domain-containing protein	-
J1F19_RS16490	3487238..3487606	+	369	WP_001295723.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J1F19_RS16495	3487696..3488070	+	375	WP_000854765.1	TA system toxin CbtA family protein	Toxin
J1F19_RS16500	3488067..3488558	+	492	WP_001586604.1	hypothetical protein	-
J1F19_RS16505	3488575..3488751	+	177	WP_001586605.1	DUF957 domain-containing protein	-
J1F19_RS16510	3488851..3489009	+	159	WP_001467148.1	hypothetical protein	-
J1F19_RS16515	3490010..3492046	+	2037	Protein_3221	hypothetical protein	-
J1F19_RS16520	3492445..3492624	-	180	WP_001315171.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13970.98 Da
• Isoelectric Point: 7.2909

>T196381 WP_000854765.1 NZ_CP071439:3487696-3488070 [Escherichia coli]
MKTLPDTHVREVSCCPSPVTIWQTLLTRLLDQHYSLTLNDTPFVDERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGASSQLINSIDILRARRATGLMTRHNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T196381 NZ_CP095109:c1063565-1063377 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTATCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13592.40 Da
• Isoelectric Point: 6.6255

>AT196381 WP_001295723.1 NZ_CP071439:3487238-3487606 [Escherichia coli]
VSDTLSGTTHPDDNDDHPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGRFRDADAYPLDQAFPLLMKQLKLMLTSG
ELNPRHQHTVTLYAKGLTCEADTLGSCGYVYLAVYPTPETKK

Nucleotide

• Length: 369 bp

>AT196381 NZ_CP095109:c1063854-1063594 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATCGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A7ZVJ9

Antitoxin

• AlphaFold DB: S1NM52