Detailed information of TA system TA196379

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): chpSB/PRK09812-ChpS
• Location: 3394630..3395225
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: chpB
• Uniprot ID: V0SXT4
• Locus tag: J1F19_RS16035
• Protein ID: WP_000239581.1
• Coordinates: 3394875..3395225 (+)
• Length: 117 a.a.

Antitoxin (Protein)

• Gene name: chpS
• Uniprot ID: L4JJX7
• Locus tag: J1F19_RS16030
• Protein ID: WP_001223213.1
• Coordinates: 3394630..3394881 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS16020	3390295..3394074	+	3780	WP_001586589.1	autotransporter assembly complex protein TamB	-
J1F19_RS16025	3394077..3394418	+	342	WP_001219160.1	gamma-glutamylcyclotransferase	-
J1F19_RS16030	3394630..3394881	+	252	WP_001223213.1	type II toxin-antitoxin system antitoxin ChpS	Antitoxin
J1F19_RS16035	3394875..3395225	+	351	WP_000239581.1	endoribonuclease toxin ChpB	Toxin
J1F19_RS16040	3395304..3395834	-	531	WP_000055075.1	inorganic diphosphatase	-
J1F19_RS16045	3396144..3397100	+	957	WP_000265933.1	galactofuranose ABC transporter substrate-binding protein YtfQ	-
J1F19_RS16050	3397240..3398742	+	1503	WP_000210560.1	sugar ABC transporter ATP-binding protein	-
J1F19_RS16055	3398756..3399778	+	1023	WP_001586590.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (8-114)

Antitoxin

• SpoVT-AbrB domain (6-49)

Sequences

Toxin

Protein

• Length: 117 a.a.
• Molecular weight: 12453.41 Da
• Isoelectric Point: 6.2206

>T196379 WP_000239581.1 NZ_CP071439:3394875-3395225 [Escherichia coli]
MVKKSEFERGDIVLVGFDPASGHEQQGAGRPALVLSVQAFNQLGMTLVAPITQGGNFARYAGFSVPLHCEEGDVHGVVLV
NQVRMMDLRARLAKRIGLAAGEVVEEALLRLQAVVE

Nucleotide

• Length: 351 bp

>T196379 NZ_CP095109:707814-707966 [Staphylococcus aureus]
ATGTCTAAAGACAAAGATCCAAAATTAAATTATCATGAAGAAGAAAACAGTATGGTAACGGATTTTGAAGATTTAAAAGA
ATTAGGTAAAGAAATGGAACAAATTTCTGATCAAAATGATCAAGAAAAGAATTCTGAAGAAGACAGTCAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9331.64 Da
• Isoelectric Point: 4.6509

>AT196379 WP_001223213.1 NZ_CP071439:3394630-3394881 [Escherichia coli]
MRITIKRWGNSSGMVIPNVVMKELNLRPGQSVEAQVSNNQLILTPISRRYSLDELLAQCDMNATELSEQDVWGKSTPAGD
EIW

Nucleotide

• Length: 252 bp

>AT196379 NZ_CP095109:707190-707789 [Staphylococcus aureus]
ATGGCAATGAACTTTAAAGTCTTTGACAATAGTCAACTTGTGGCAGAATATGCTGCTGATATTATTAGAAAGCAATTTAA
CAATAATCCTACTACAATTGCAGGTTTTCATTTAGATACAGATCAAGCGCCAGTACTAGATGAATTAAAGAAAAATGTTG
AAAAACATGCTGTTGATTTTAGCCAAATAAATATTTTAGATTATGACGATAAAAAATCATATTTCGAAGCGTTAGGTGTA
CCAGCAGGTCAAGTTTATCCAATTGCTTATGAAAAAGATGCAATCGAATTAATCGCTGATAAGATTAAAACTAAAGAAAA
TAAAGGGAAATTAACATTACAAGTTGTTTCTATCGATGAGCAAGGTAAGTTAAATGTTAGTATTCGTCAAGGACTAATGG
AAGCAAGAGAAATTTTCTTAGTAGTAACTGGTACTAATAAACGAGATGTAGTTGAAAAATTATATCAAGAAAATGGTAAA
ACAAGCTTCGAACCAGCCGATTTAAAAGCACATAGAATGGTAAATGTTATTCTTGATAAAGAAGCGGCTGCAGGTTTACC
TGAAGATGTTAAAGCTTACTTTACGTCACGCTTTGCTTAA

Structures

Toxin

• AlphaFold DB: V0SXT4

Antitoxin

• AlphaFold DB: L4JJX7