Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type V Classification (family/domain) ghoTS/ghoT-GhoS
Location 3295175..3295672 Replicon chromosome
Accession NZ_CP071439
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name ghoT Uniprot ID L4JJG6
Locus tag J1F19_RS15530 Protein ID WP_001173343.1
Coordinates 3295499..3295672 (+) Length 58 a.a.

Antitoxin (Protein)


Gene name ghoS Uniprot ID L4JK92
Locus tag J1F19_RS15525 Protein ID WP_000398619.1
Coordinates 3295175..3295471 (+) Length 99 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS15505 3291904..3292623 - 720 WP_000611288.1 two-component system response regulator DcuR -
J1F19_RS15510 3292620..3294251 - 1632 WP_001216477.1 sensor histidine kinase -
J1F19_RS15515 3294432..3294662 + 231 WP_000373291.1 (4Fe-4S)-binding protein -
J1F19_RS15520 3294674..3294946 + 273 WP_000405647.1 N-acetyltransferase -
J1F19_RS15525 3295175..3295471 + 297 WP_000398619.1 type V toxin-antitoxin system endoribonuclease antitoxin GhoS Antitoxin
J1F19_RS15530 3295499..3295672 + 174 WP_001173343.1 type V toxin-antitoxin system toxin GhoT Toxin
J1F19_RS15535 3295790..3297307 - 1518 WP_000003806.1 lysine--tRNA ligase -
J1F19_RS15540 3297544..3299001 - 1458 WP_000856848.1 dipeptide/tripeptide permease DtpC -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin GhoT/OrtT

(4-57)

Antitoxin

Endoribonuclease antitoxin GhoS

(5-94)


Sequences

Toxin        


Download         Length: 58 a.a.        Molecular weight: 6555.06 Da        Isoelectric Point: 10.2288

>T196378 WP_001173343.1 NZ_CP071439:3295499-3295672 [Escherichia coli]
MALFSKILIFYVIGVNISFVIIWFISHEKTHIRLLSAFLVGITWPMSLPVALLFSLF

Download         Length: 174 bp

>T196378 NZ_CP095107:c1131853-1131392 [Staphylococcus delphini]
ATGGGATTATACGAAGAAACTTTAATACAACATGACTATATAGACGTAAGAGAGGCGAATGTACTTCCTGATGATTTAGA
TGGAGTCTGGCTAGGTGATCTAATATTAATTAAACGTAACCTACCAGAAAAGAAGAAAGTCGAAGTCCTTTTTGAAGAAT
TAGCACATCACAAACTTACATACGGTGACATCACCAATCAAAAACAGTTCAACAACCGAAAATTCGAAAATTACGCTAGA
CGCTATTCATACGAAAAGTCTATGCCCCTATCGGGTATAGTCCAGGCGTTTGAGCAAGGGGTACATAATCTGTACGAGCT
TGCTAATTTTTTTGAGATTTCAGAAGGTCACGTACTAGATTGTATTGAGCATTATAAAAAGAAGTATGGTTTACAAGTTA
AATATGGCAAGTATCTAATTAGATTCGAACCGTTAACTATATATAAAGATTTAGCTGATTAA

Antitoxin


Download         Length: 99 a.a.        Molecular weight: 11467.97 Da        Isoelectric Point: 4.1705

>AT196378 WP_000398619.1 NZ_CP071439:3295175-3295471 [Escherichia coli]
MEGKNKFNTYVVSFDYPSSYSSVFLRLRSLMYDMNFSSIVADEYGIPRQLNENSFAITTSLAASEIEDLIRLKCLDLPDI
DFDLNIMTVDDYFRQFYK

Download         Length: 297 bp

>AT196378 NZ_CP095107:c1132195-1131866 [Staphylococcus delphini]
ATGAGAAACAATGATGAAATAATCACAATAATCAAAGCATCTATGAAAAAACAAAATTTGTCGCTTAGTGAATTGGCTCG
TCGTGTAGGAGTTGCTAAATCTGCTGTGTCACGTTATTTAAATTTAACAAGAGAATTTCCATTAAATCGAGCAGAAGATT
TTGCAAAAGCGCTTAGTATCAGTACAGAATATTTACTTGGTTTTGAAAAAAGTGAACAAAAACAAGAACAACCACAGCAC
CGCGCAGCTCATTTGGAAGGAGAGCTGACTGATGAAGAGTGGCAACGTGTGTTAGATTATGCAGACTATATTAGAAGCAA
ACGTAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U9QI41

Antitoxin

Source ID Structure
PDB 2LLZ
AlphaFold DB A0A7U9QBH6

References