Detailed information of TA system TA196377

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/HicA-HicB
• Location: 2662740..2663352
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: U9YXE2
• Locus tag: J1F19_RS12725
• Protein ID: WP_000833473.1
• Coordinates: 2663167..2663352 (-)
• Length: 62 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: L4IWR9
• Locus tag: J1F19_RS12720
• Protein ID: WP_000499744.1
• Coordinates: 2662740..2663150 (-)
• Length: 137 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS12705	2658252..2659067	-	816	WP_000891838.1	AraC family transcriptional regulator	-
J1F19_RS12710	2659293..2660693	+	1401	WP_000204822.1	MFS transporter	-
J1F19_RS12715	2660704..2662668	+	1965	WP_001026868.1	glycoside hydrolase family 127 protein	-
J1F19_RS12720	2662740..2663150	-	411	WP_000499744.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J1F19_RS12725	2663167..2663352	-	186	WP_000833473.1	type II toxin-antitoxin system HicA family toxin	Toxin
J1F19_RS12730	2663621..2665159	-	1539	WP_000183978.1	aldehyde dehydrogenase AldB	-
J1F19_RS12735	2665360..2666511	-	1152	WP_000741501.1	L-threonine dehydrogenase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (4-59)

Antitoxin

• HicB-like antitoxin of toxin-antitoxin system (3-129)

Sequences

Toxin

Protein

• Length: 62 a.a.
• Molecular weight: 6800.89 Da
• Isoelectric Point: 11.7053

>T196377 WP_000833473.1 NZ_CP071439:c2663352-2663167 [Escherichia coli]
VKSADVIAILKQHGWEHIRTRGSHHQFRHPVHPGLVTVPHPKKDIKPGTLAQIGRQAGLTF

Nucleotide

• Length: 186 bp

>T196377 NZ_CP095107:916279-916632 [Staphylococcus delphini]
ATGAAGCGCGGAGACGTCTATTTAGCAGATTTATCACCAGTACAGGGCTCTGAACAGGGGGGAGTAAGGCCCGTGGTCAT
TATCCAAAATGACACTGGTAATAAGTATAGTCCCACTGTGATTGTAGCCGCAATTACTGGTCGGATTAATAAGGCAAAGA
TACCAACGCATGTCGAAATCGAAAAAAATAAATATAAGCTCGATAAAGATTCTGTTATTTTACTAGAACAAATTCGAACA
GTCGATAAGAAACGATTAAAAGAAAAGTTAACGTTTTTGTCTGAGAAGAAGATGAAAGAAGTTAATAATGCGATTGGTAT
TAGTTTAGGACTGCACATGAATCATCATAAATAG

Antitoxin

Protein

• Length: 137 a.a.
• Molecular weight: 15241.15 Da
• Isoelectric Point: 4.5486

>AT196377 WP_000499744.1 NZ_CP071439:c2663150-2662740 [Escherichia coli]
MFYPAYIHSDPDGSASGFFPDVPGCYFAGDTLDNAFQDARSALVAHFETLCEMNEELPLPGSVETHLVQRAQDFIGGQWL
LVDINMNQFEGRAERINITMPKRLLNKIDTYVRNNPDYANRSAFLAEAARRVLPGV

Nucleotide

• Length: 411 bp

>AT196377 NZ_CP095107:916108-916278 [Staphylococcus delphini]
ATGTCAGCATTCAATCAAACAAGATTTAAAAATTTAGAACAGTCGTTAAAAGAAGGTTATGTACAGATGGCAGATTTAAA
TCTCTCCCTAGCCACTGAGGCGTATCCAGTTGAATGTGAAGCTTGCGATTGTAATGAATCACACTTGACAACTGATTATA
AGGAAGACTAA

Structures

Toxin

• AlphaFold DB: U9YXE2

Antitoxin

• AlphaFold DB: L4IWR9