Detailed information of TA system TA196374

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 1974008..1974842
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: A0A8E0IX31
• Locus tag: J1F19_RS09415
• Protein ID: WP_000854689.1
• Coordinates: 1974465..1974842 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: A0A8E0IVP8
• Locus tag: J1F19_RS09410
• Protein ID: WP_001285599.1
• Coordinates: 1974008..1974388 (+)
• Length: 127 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS09375	1970095..1970550	+	456	WP_001309747.1	hypothetical protein	-
J1F19_RS09380	1970651..1970782	+	132	WP_106108514.1	DUF905 family protein	-
J1F19_RS09385	1970961..1971782	+	822	WP_001234555.1	DUF945 domain-containing protein	-
J1F19_RS09390	1972046..1972519	+	474	WP_001538139.1	antirestriction protein	-
J1F19_RS09395	1972535..1973011	+	477	WP_001186761.1	RadC family protein	-
J1F19_RS09400	1973074..1973295	+	222	WP_000692315.1	DUF987 domain-containing protein	-
J1F19_RS09405	1973314..1973958	+	645	WP_000086757.1	hypothetical protein	-
J1F19_RS09410	1974008..1974388	+	381	WP_001285599.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J1F19_RS09415	1974465..1974842	+	378	WP_000854689.1	TA system toxin CbtA family protein	Toxin
J1F19_RS09420	1974839..1975327	+	489	WP_000761698.1	hypothetical protein	-
J1F19_RS09425	1975344..1975541	+	198	WP_000839250.1	DUF957 domain-containing protein	-
J1F19_RS09430	1975626..1976471	+	846	WP_001446750.1	DUF4942 domain-containing protein	-
J1F19_RS09435	1976540..1976935	+	396	WP_000208381.1	type IV toxin-antitoxin system AbiEi family antitoxin domain-containing protein	-
J1F19_RS09440	1976928..1977861	+	934	Protein_1851	nucleotidyl transferase AbiEii/AbiGii toxin family protein	-
J1F19_RS09445	1978293..1978448	+	156	WP_000729638.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	1951288..1978448	27160
	-	flank	IS/Tn	-	-	1978293..1978448	155

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14042.07 Da
• Isoelectric Point: 9.1510

>T196374 WP_000854689.1 NZ_CP071439:1974465-1974842 [Escherichia coli]
MKTLPDTHVRAASRCPSPVTIWQTLLTRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SAGAPSQLINSIDILRARRATGLMTRNNYRMVNNITQGKHPEAKR

Nucleotide

• Length: 378 bp

>T196374 NZ_CP095104:c1801061-1800711 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAACTGACTTATCTTTCAGATGAGAAGATGAAAGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 127 a.a.
• Molecular weight: 13984.78 Da
• Isoelectric Point: 5.0823

>AT196374 WP_001285599.1 NZ_CP071439:1974008-1974388 [Escherichia coli]
MSDTLPGTTLPDDNKDRPWWGLPCTVTPCFGARLLQEGNRLHYLADRAGIRGRFSNADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCDADTLGSCGYVYLAVYPATETESNPPE

Nucleotide

• Length: 381 bp

>AT196374 NZ_CP095104:c1801225-1801058 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA