Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 1869569..1870223 Replicon chromosome
Accession NZ_CP071439
Organism Escherichia coli strain EcPNK004

Toxin (Protein)


Gene name cptA Uniprot ID S1PAM6
Locus tag J1F19_RS08880 Protein ID WP_000244781.1
Coordinates 1869569..1869976 (-) Length 136 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID S1PPB1
Locus tag J1F19_RS08885 Protein ID WP_000354046.1
Coordinates 1869957..1870223 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F19_RS08860 1865526..1867259 - 1734 WP_001587399.1 single-stranded-DNA-specific exonuclease RecJ -
J1F19_RS08865 1867265..1867975 - 711 WP_000715213.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J1F19_RS08870 1868000..1868896 - 897 WP_001587400.1 site-specific tyrosine recombinase XerD -
J1F19_RS08875 1869008..1869529 + 522 WP_001055874.1 flavodoxin FldB -
J1F19_RS08880 1869569..1869976 - 408 WP_000244781.1 protein YgfX Toxin
J1F19_RS08885 1869957..1870223 - 267 WP_000354046.1 FAD assembly factor SdhE Antitoxin
J1F19_RS08890 1870466..1871446 + 981 WP_001587401.1 tRNA-modifying protein YgfZ -
J1F19_RS08895 1871523..1872182 - 660 WP_000250274.1 hemolysin III family protein -
J1F19_RS08900 1872346..1872657 - 312 WP_001182957.1 N(4)-acetylcytidine aminohydrolase -
J1F19_RS08905 1872702..1874135 + 1434 WP_001587402.1 6-phospho-beta-glucosidase BglA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 136 a.a.        Molecular weight: 16061.99 Da        Isoelectric Point: 11.5202

>T196373 WP_000244781.1 NZ_CP071439:c1869976-1869569 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDSGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Download         Length: 408 bp

>T196373 NZ_CP095103:c10662-10396 [Staphylococcus simulans]
ATGAGTTATTACAGTGTCTTGATTAAAAATTCAGCTAAAAGTGATTTGAAAAAAATAAACAAATCGCACCTCAAAGAACA
ATTTTTAAATGTTGTTGAAACTTTAAAAATCAATCCTTATGAACCTAGTCAATCTTTTGAAAAGTTGCAACCAAAGCATT
TAGGACGTTATTCAAGACGTATTAATCATCAGCATAGAATTGTTTATACAGTTGATGATGAAAAGAAGGAAGTTTATATA
TTTTCTGCTTGGTCGCATTATGAATAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10547.12 Da        Isoelectric Point: 5.1599

>AT196373 WP_000354046.1 NZ_CP071439:c1870223-1869957 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAELEMMVRLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196373 NZ_CP095103:c10919-10662 [Staphylococcus simulans]
ATGAATATTAAAACACCTACAAACGCTAGAAAAGAATTTTATGAATTATTAAAACATGTAAATGTTAATCATGAGCCTGT
TATTATAAGTGGTTCTAAAAGTGAAAATAACGCTGTTATTGTAAGTCAAAAAGACTGGGATAGCATTCAAGAAACTTTAT
ACCTTGAATCAGTTGGCGTAATGGATAAAGTGAGAGAACGTGAAAAAGACAGTAGTAGCTATACAAATATCGATGATTTA
GATTGGAGTAACTTGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PAM6

Antitoxin

Source ID Structure
PDB 6B58
PDB 1X6I
PDB 1X6J
PDB 6C12
AlphaFold DB A0A7U9QD57

References