Detailed information of TA system TA196372

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 871511..872342
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: S1P968
• Locus tag: J1F19_RS04325
• Protein ID: WP_000854814.1
• Coordinates: 871968..872342 (+)
• Length: 125 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: B3Y195
• Locus tag: J1F19_RS04320
• Protein ID: WP_001285585.1
• Coordinates: 871511..871879 (+)
• Length: 123 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS04285	867497..868339	+	843	Protein_843	hypothetical protein	-
J1F19_RS04290	868415..868870	+	456	WP_000581504.1	hypothetical protein	-
J1F19_RS04295	868949..869182	+	234	WP_001119729.1	DUF905 family protein	-
J1F19_RS04300	869282..870100	+	819	WP_021538423.1	DUF945 domain-containing protein	-
J1F19_RS04305	870182..870661	+	480	WP_000860087.1	antirestriction protein	-
J1F19_RS04310	870677..871153	+	477	WP_001186726.1	RadC family protein	-
J1F19_RS04315	871216..871437	+	222	WP_000692323.1	DUF987 domain-containing protein	-
J1F19_RS04320	871511..871879	+	369	WP_001285585.1	type IV toxin-antitoxin system toxin CbtA	Antitoxin
J1F19_RS04325	871968..872342	+	375	WP_000854814.1	type IV toxin-antitoxin system toxin CbtA	Toxin
J1F19_RS04330	872339..872533	+	195	WP_000988600.1	hypothetical protein	-
J1F19_RS04335	872546..872659	+	114	WP_001161660.1	DUF957 domain-containing protein	-
J1F19_RS04340	872948..873076	-	129	Protein_854	transposase domain-containing protein	-
J1F19_RS04345	873148..873330	+	183	WP_032140263.1	ethanolamine utilization protein	-
J1F19_RS04350	873431..873760	-	330	WP_000450409.1	DUF496 family protein	-
J1F19_RS04355	873932..874990	-	1059	WP_001200891.1	FUSC family protein	-
J1F19_RS04360	875188..875661	-	474	WP_001105389.1	DNA gyrase inhibitor SbmC	-
J1F19_RS04365	875780..876946	-	1167	WP_000830150.1	serine-type D-Ala-D-Ala carboxypeptidase DacD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (18-117)

Sequences

Toxin

Protein

• Length: 125 a.a.
• Molecular weight: 13898.94 Da
• Isoelectric Point: 7.8522

>T196372 WP_000854814.1 NZ_CP071439:871968-872342 [Escherichia coli]
MKTLPVLPGQAASSRPSPVEIWQILLSRLLDQHYGLTLNDTPFADERVIEQHIEAGISLCDAVNFLVEKYALVRTDQPGF
SACTRSQLINSIDILRARRATGLMTRDNYRTVNNITLGKYPEAK

Nucleotide

• Length: 375 bp

>T196372 NZ_CP095102:986494-986844 [Staphylococcus simulans]
ATGATGAGGAGAGGCGATGTTTATTTAGCTGATTTATCACCAGTGCAAGGTTCTGAACAAGGGGGAGTTAGACCTGTTGT
AATTATACAAAATGACACTGGCAATAAGTACAGTCCGACGGTAATTGTAGCCGCGATTACTGGGCGTATTAATAAGGCGA
AAATTCCAACACATGTTGAAATTGAAAAGAGTAAATACAAATTAGACAAAGATTCTGTTATATTACTTGAACAGATTAGA
ACAGTTGATAAAAAACGTTTGAAAGAGAAATTGACTTATCTTTCAGATGAGAAGATGAAAGAAATTGATAACGCGATTGG
AATTAGTTTAGGCTTGACACATAAAAATTAG

Antitoxin

Protein

• Length: 123 a.a.
• Molecular weight: 13593.48 Da
• Isoelectric Point: 6.3139

>AT196372 WP_001285585.1 NZ_CP071439:871511-871879 [Escherichia coli]
VSDTLPGTTLPDDNHDRPWWGLPCTVTPCFGARLVQEGNRLHYLADRAGIRGLFSDADAYHLDQAFPLLMKQLELMLTSG
ELNPRHQHTVTLYAKGLTCKADTLSSCGYVYLAVYPTPEMKN

Nucleotide

• Length: 369 bp

>AT196372 NZ_CP095102:986330-986497 [Staphylococcus simulans]
ATGTCAACTTTTAATCAGAATAGAAGCTATAGTTTGGAACAGTCATTAAAAGAAGGCTATTCACAAATGGCTGATTTGAA
TCTCTCCCTAGCAACGGAGGCATTTTCAGTGGAATGTGAAGCCTGTGATTGCAATGAATCTTATCTTACATTTGATAAGG
ATGAATGA

Structures

Toxin

• AlphaFold DB: A0A829FY50

Antitoxin

• AlphaFold DB: A0A7U9LW60