Detailed information of TA system TA196362

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/UPF0150(antitoxin)
• Location: 221129..221767
• Replicon: chromosome
• Accession: NZ_CP071439
• Organism: Escherichia coli strain EcPNK004

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: D3GRG9
• Locus tag: J1F19_RS01145
• Protein ID: WP_001314712.1
• Coordinates: 221129..221305 (+)
• Length: 59 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J1F19_RS01150
• Protein ID: WP_077248684.1
• Coordinates: 221351..221767 (+)
• Length: 139 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F19_RS01125	216751..217962	-	1212	WP_071593600.1	BenE family transporter YdcO	-
J1F19_RS01130	218015..218551	+	537	WP_000429149.1	DNA-binding transcriptional regulator SutR	-
J1F19_RS01135	218624..220585	+	1962	WP_001587031.1	23S rRNA 5-hydroxycytidine C2501 synthase	-
J1F19_RS01140	220677..220907	-	231	WP_000494241.1	YncJ family protein	-
J1F19_RS01145	221129..221305	+	177	WP_001314712.1	type II toxin-antitoxin system mRNA interferase toxin HicA	Toxin
J1F19_RS01150	221351..221767	+	417	WP_077248684.1	type II toxin-antitoxin system antitoxin HicB	Antitoxin
J1F19_RS01155	221846..223252	+	1407	WP_000760623.1	PLP-dependent aminotransferase family protein	-
J1F19_RS01160	223497..224642	+	1146	WP_000047443.1	ABC transporter substrate-binding protein	-
J1F19_RS01165	224660..225673	+	1014	WP_000220407.1	ABC transporter ATP-binding protein	-
J1F19_RS01170	225674..226615	+	942	WP_001587036.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (5-57)

Antitoxin

• Cro/C1-type helix-turn-helix domain (87-134)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6746.81 Da
• Isoelectric Point: 11.8888

>T196362 WP_001314712.1 NZ_CP071439:221129-221305 [Escherichia coli]
VKQSEFRRWLESQGVNVANGSNHLKLRFHGRRSVMPRHPSEEIKEPLRKAILKQLGLS

Nucleotide

• Length: 177 bp

>T196362 NZ_CP095095:c2723-2451 [Flavonifractor plautii]
ATGCTGACGATTAAGTATCAGACTGCCTTTAAGAAAGACTACAAAAGAATTGTAAGGCGTGGCTATGATGTGCGCTTACT
GGAAAAGGTGATTGGGTTGCTGGCTGAGCAGAAACCATTGCCGGAGCAGTATCACGATCACAACTTGACAGGGAATTATG
CTGGCTGCCGTGAGTGCCACATTACCCCGGACTGGCTGCTGATCTATGAAGTGGATGGAAACGAGCTGATTTTATATCTC
ACCCGGACAGGATCTCATAGCGATTTATTTTAG

Antitoxin

Protein

• Length: 139 a.a.
• Molecular weight: 15232.60 Da
• Isoelectric Point: 4.7286

>AT196362 WP_077248684.1 NZ_CP071439:221351-221767 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSDITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Nucleotide

• Length: 417 bp

>AT196362 NZ_CP095095:c2982-2710 [Flavonifractor plautii]
ATGGCTAACACAAATATCAATATTCGTATGGATACAGACTTGAAAAAGCAGTTTGAAGCGTTTTGCGCAGACATGGGAAT
GACTATGACAACGGCATTTAATGTTTTTGCGAGAAAGGCTGTCCGAGAGTATAGAATACCATTTGAAGTTGGCGGAGAGG
TACCGAATGACGACACTAAAAAGGCTATGGAGGACGCGCAAAAAGGGATCGGCATGAGCAAGGGGTTTTCTTCTGTAGAA
GAACTCATGGAGGAGTTAAATGCTGACGATTAA

Structures

Toxin

• AlphaFold DB: D3GRG9