Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 3368442..3369060 Replicon chromosome
Accession NZ_CP071436
Organism Escherichia coli strain EcPNK006

Toxin (Protein)


Gene name Hha Uniprot ID H5UYE2
Locus tag J1137_RS16330 Protein ID WP_001291435.1
Coordinates 3368842..3369060 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID S1PTH5
Locus tag J1137_RS16325 Protein ID WP_000344800.1
Coordinates 3368442..3368816 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1137_RS16315 3363531..3364724 + 1194 WP_001295324.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J1137_RS16320 3364747..3367896 + 3150 WP_001132469.1 multidrug efflux RND transporter permease subunit -
J1137_RS16325 3368442..3368816 + 375 WP_000344800.1 Hha toxicity modulator TomB Antitoxin
J1137_RS16330 3368842..3369060 + 219 WP_001291435.1 hemolysin expression modulator Hha Toxin
J1137_RS16335 3369232..3369783 + 552 WP_000102564.1 maltose O-acetyltransferase -
J1137_RS16340 3369899..3370369 + 471 WP_000136192.1 YlaC family protein -
J1137_RS16345 3370562..3371259 + 698 Protein_3194 IS1-like element IS1A family transposase -
J1137_RS16350 3371310..3372860 + 1551 WP_001310610.1 cyclic-guanylate-specific phosphodiesterase PdeB -
J1137_RS16355 3372902..3373255 - 354 Protein_3196 DUF1428 family protein -
J1137_RS16365 3373634..3373945 + 312 WP_000409911.1 MGMT family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-117)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8628.00 Da        Isoelectric Point: 8.9008

>T196350 WP_001291435.1 NZ_CP071436:3368842-3369060 [Escherichia coli]
MSEKPLTKTDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPSSVWKFIR

Download         Length: 219 bp

>T196350 NZ_CP095089:10261-10542 [Escherichia coli]
ATGACCTATGAACTGGCTTTTGACCGCAGAGCACTGAAGGAATGGCAGAAACTCGGCCACACCATCCGTGAACAATTCAA
AAAGAAGCTGGCAGAACGGCTGGAAAATCCACGCGTACCCGCAGCCCGGTTACATGGTCATGCTGATCGCTATAAAATCA
AACTTCGTGCATCTGGCTACAGACTTGTATATCAAGTCATTGATGAGAAAGTCGTTTTACTCGTTATTGCTGTTGGAAGA
AGGGAAAGCAGCGAAGTTTATCAGATTGCAGATATGCGCTGA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14557.39 Da        Isoelectric Point: 4.7395

>AT196350 WP_000344800.1 NZ_CP071436:3368442-3368816 [Escherichia coli]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAINLQLNELIEHIATFALNYKIKYNEDNKLIEQIDEYL
DDTFMLFSSYGINMQDLQKWRKSGNRLFRCFVNATKENPASLSC

Download         Length: 375 bp

>AT196350 NZ_CP095089:10020-10271 [Escherichia coli]
ATGAGCTATCAAATTCTGACAACCACAGCGGCCAGTATTACTGACCTGAAAAAAAATCCTATGGGAACCGTAGCTGAAGG
TGAAGGGGACGCTGTTGCGATCCTGAACCGAAACGAACCGGCGTTCTATTGCGTTCCACCAAAACTTTACGCCTACTATC
GGGAACTCGCTGAAGATGCTGAGTTAAACGCTGTTGCTGATGAGCGCATGAAAAACCCGGAAATTGTGAAGGTTAACCTG
GATGACCTATGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 2MW2
PDB 1JW2

Antitoxin

Source ID Structure
AlphaFold DB A0A7U9QBQ5

References