Detailed information of TA system TA196349

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): relBE/RelE-HTH_XRE
• Location: 2998881..2999586
• Replicon: chromosome
• Accession: NZ_CP071436
• Organism: Escherichia coli strain EcPNK006

Toxin (Protein)

• Gene name: relE
• Uniprot ID: S1F406
• Locus tag: J1137_RS14560
• Protein ID: WP_000539521.1
• Coordinates: 2998881..2999267 (+)
• Length: 129 a.a.

Antitoxin (Protein)

• Gene name: relB
• Uniprot ID: -
• Locus tag: J1137_RS14565
• Protein ID: WP_001280945.1
• Coordinates: 2999257..2999586 (+)
• Length: 110 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1137_RS14540	2994885..2995511	+	627	WP_001295292.1	glutathione S-transferase GstB	-
J1137_RS14545	2995508..2996623	-	1116	Protein_2848	aldose sugar dehydrogenase YliI	-
J1137_RS14550	2996734..2997117	-	384	WP_000497137.1	biofilm formation regulator BssR	-
J1137_RS14555	2997330..2998655	+	1326	WP_000049367.1	30S ribosomal protein S12 methylthiotransferase RimO	-
J1137_RS14560	2998881..2999267	+	387	WP_000539521.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J1137_RS14565	2999257..2999586	+	330	WP_001280945.1	DNA-binding transcriptional regulator	Antitoxin
J1137_RS14570	2999656..3000984	-	1329	WP_000086877.1	GGDEF domain-containing protein	-
J1137_RS14575	3000992..3003340	-	2349	WP_134778638.1	EAL domain-containing protein	-
J1137_RS14580	3003518..3004429	-	912	WP_001236042.1	glutathione ABC transporter permease GsiD	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (1-124)

Antitoxin

• Cro/C1-type helix-turn-helix domain (53-94)

Sequences

Toxin

Protein

• Length: 129 a.a.
• Molecular weight: 14293.45 Da
• Isoelectric Point: 9.9296

>T196349 WP_000539521.1 NZ_CP071436:2998881-2999267 [Escherichia coli]
MGVYKARRFSQSTKKLGIHDKVLMAAAEEVMQGIWEADLGSGVIKKRLPLQQGKSGGARTIIFFKSANHVFFYDGWSKSG
LSSKGTKEIEDDELAAYKKMANAFLAFSNKQIEDLIETGFLIEVKNER

Nucleotide

• Length: 387 bp

>T196349 NZ_CP095088:c4236111-4235938 [Escherichia coli]
ATGGCACTATTCTCTAAAATATTAATTTTTTATGTGATTGGTGTGAACATATCCTTTGTCATTATCTGGTTTATCTCACA
TGAGAAAACACATATTCGTTTACTTAGTGCATTCCTGGTCGGAATAACCTGGCCAATGAGTCTGCCTGTGGCATTACTTT
TTTCTCTCTTTTAG

Antitoxin

Protein

• Length: 110 a.a.
• Molecular weight: 11982.08 Da
• Isoelectric Point: 10.0101

>AT196349 WP_001280945.1 NZ_CP071436:2999257-2999586 [Escherichia coli]
MSDKLQMMLEDAKELNALGLMSDSEVDAIAVMVKAREVSARIAKVKSMTGEEIKAIRTRYGMSQSVLAHTMGMSKESVSK
WERNEIKPSGPALRILNTLAIKGPEVFAK

Nucleotide

• Length: 330 bp

>AT196349 NZ_CP095088:c4236435-4236139 [Escherichia coli]
ATGGAAGGTAAAAACAAGTTCAATACTTATGTTGTTTCTTTTGATTATCCATCATCTTATTCCTCAGTGTTCTTAAGGTT
AAGATCATTGATGTATGATATGAATTTCTCCTCTATCGTGGCTGATGAATATGGGATACCACGACAATTGAATGAAAACT
CCTTCGCAATAACGACATCGTTAGCCGCAAGTGAAATCGAAGATTTAATCAGGCTTAAATGCTTAGACTTACCGGATATT
GACTTTGACCTCAACATTATGACAGTTGATGACTATTTCCGTCAGTTTTACAAGTAG

Structures

Toxin

• AlphaFold DB: A0A829GCT6