Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) hicAB/UPF0150(antitoxin)
Location 2343100..2343738 Replicon chromosome
Accession NZ_CP071436
Organism Escherichia coli strain EcPNK006

Toxin (Protein)


Gene name hicA Uniprot ID S1F9G9
Locus tag J1137_RS11250 Protein ID WP_000813794.1
Coordinates 2343562..2343738 (-) Length 59 a.a.

Antitoxin (Protein)


Gene name hicB Uniprot ID -
Locus tag J1137_RS11245 Protein ID WP_001270286.1
Coordinates 2343100..2343516 (-) Length 139 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1137_RS11225 2338252..2339193 - 942 WP_001251313.1 ABC transporter permease -
J1137_RS11230 2339194..2340207 - 1014 WP_000220399.1 ABC transporter ATP-binding protein -
J1137_RS11235 2340225..2341370 - 1146 WP_000047421.1 ABC transporter substrate-binding protein -
J1137_RS11240 2341615..2343021 - 1407 WP_000760626.1 PLP-dependent aminotransferase family protein -
J1137_RS11245 2343100..2343516 - 417 WP_001270286.1 type II toxin-antitoxin system antitoxin HicB Antitoxin
J1137_RS11250 2343562..2343738 - 177 WP_000813794.1 type II toxin-antitoxin system mRNA interferase toxin HicA Toxin
J1137_RS11255 2343960..2344190 + 231 WP_000494244.1 YncJ family protein -
J1137_RS11260 2344282..2346243 - 1962 WP_001301045.1 23S rRNA 5-hydroxycytidine C2501 synthase -
J1137_RS11265 2346316..2346852 - 537 WP_000429155.1 DNA-binding transcriptional regulator SutR -
J1137_RS11270 2346905..2348119 + 1215 WP_071607224.1 BenE family transporter YdcO -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

HicA mRNA interferase family

(5-57)

Antitoxin

Cro/C1-type helix-turn-helix domain

(91-134)


Sequences

Toxin        


Download         Length: 59 a.a.        Molecular weight: 6749.83 Da        Isoelectric Point: 11.2298

>T196348 WP_000813794.1 NZ_CP071436:c2343738-2343562 [Escherichia coli]
VKQSEFRRWLESQGVDVANGSNHLKLRFHGRRSVMPRHPCDEIKEPLRKAILKQLGLS

Download         Length: 177 bp

>T196348 NZ_CP095088:c4131775-4131425 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GTGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTT
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGATGAGGTGGTGGAAGAGGC
GTTATTACGCTTGCAGGCGGTGGTGGAATAA

Antitoxin


Download         Length: 139 a.a.        Molecular weight: 15246.63 Da        Isoelectric Point: 4.7386

>AT196348 WP_001270286.1 NZ_CP071436:c2343516-2343100 [Escherichia coli]
MRYPVTLTPAPEGGYMVSFVDIPEALTQGETVAEAMEAAKDALLTAFDFYFEDNELIPLPSPLNSHDHFIEVPLSVASKV
LLLNAFLQSEITQQELARRIGKPKQEITRLFNLHHATKIDAVQLAAKALGKELSLVMV

Download         Length: 417 bp

>AT196348 NZ_CP095088:c4132020-4131769 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTGCAGGTATGGTCATTCCCAATATCGTAATGAAAGAACTTAACTTACA
GCCGGGGCAGAGCGTGGAAGCGCAGGTGAGCAACAACCAACTGATTCTGACACCCATCTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCGCGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6HPB

Antitoxin

Source ID Structure

References