Detailed information of TA system TA196317

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hicAB/-
• Location: 623421..624013
• Replicon: chromosome
• Accession: NZ_CP071405
• Organism: Pantoea deleyi strain LMG24200

Toxin (Protein)

• Gene name: hicA
• Uniprot ID: -
• Locus tag: J1C59_RS03040
• Protein ID: WP_128085149.1
• Coordinates: 623421..623675 (+)
• Length: 85 a.a.

Antitoxin (Protein)

• Gene name: hicB
• Uniprot ID: -
• Locus tag: J1C59_RS03045
• Protein ID: WP_128085148.1
• Coordinates: 623672..624013 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1C59_RS03015	618830..619834	+	1005	WP_128085153.1	phosphoadenosine phosphosulfate reductase family protein	-
J1C59_RS03020	619831..622080	+	2250	WP_208721882.1	replication endonuclease	-
J1C59_RS03025	622281..622472	+	192	WP_128085152.1	hypothetical protein	-
J1C59_RS03030	622491..622724	+	234	Protein_585	DinI-like family protein	-
J1C59_RS03035	622799..623044	+	246	WP_128085151.1	hypothetical protein	-
J1C59_RS03040	623421..623675	+	255	WP_128085149.1	type II toxin-antitoxin system HicA family toxin	Toxin
J1C59_RS03045	623672..624013	+	342	WP_128085148.1	type II toxin-antitoxin system HicB family antitoxin	Antitoxin
J1C59_RS03050	624071..625690	-	1620	WP_128085147.1	hypothetical protein	-
J1C59_RS03055	625814..626854	-	1041	WP_128085146.1	phage portal protein	-
J1C59_RS03060	626854..628617	-	1764	WP_128085145.1	terminase ATPase subunit family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	rfaE	614341..681733	67392

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HicA mRNA interferase family (23-81)

Antitoxin

• Uncharacterised protein family HicB (55-100)

Sequences

Toxin

Protein

• Length: 85 a.a.
• Molecular weight: 9496.02 Da
• Isoelectric Point: 11.3526

>T196317 WP_128085149.1 NZ_CP071405:623421-623675 [Pantoea deleyi]
MNKRHQKTLSDVFARPVSGSIKWSDIESLFIALGAEVHEREGSRIAVLLKGQKKIFHRPHPRPTTDKGAVNSIRLWLDSL
GIRP

Nucleotide

• Length: 255 bp

>T196317 NZ_CP095086:c4189141-4188827 [Escherichia coli]
ATGCAATTTACGGTATACCGCAGTCGCGGCAGGAACGCCGCGTTTCCCTTTGTTATTGATGTTACCAGCGACATTATTGG
TGAGATTAATCGCCGTATCGTTATTCCATTAACACCTATTGAACGATTTAGCCGTATTCGCCCACCAGAACGGCTTAACC
CAATCCTTTTACTGGTAGACGGCAAAGAATATGTGCTCATGACGCATGAGACTGCAACTGTTCCAGTTAACGCGCTGGGA
ACGAAATTTTGCGATGCCAGCGCGCACCGAACGTTGATTAAAGGAGCATTAGATTTTATGCTCGACGGGATTTAA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 12555.19 Da
• Isoelectric Point: 4.6212

>AT196317 WP_128085148.1 NZ_CP071405:623672-624013 [Pantoea deleyi]
MNNTLKIDGHLAVITFDPEIEMFRGEFVGLNGGADFYAYSVEELKKEGSISLAVFLEECRKDDIEPYKTFSGKVTTRLTP
ERHQALAVTAQAHGVSINELLNEGVDLVIEKHS

Nucleotide

• Length: 342 bp

>AT196317 NZ_CP095086:c4189377-4189144 [Escherichia coli]
ATGACTGCAAAACGTACCACACAAAGTGTGACCGTCACCGTCGACCGTGAGTTAGTCAATCGCGCTCGTGATGCAGGCTT
AAATATGAGCGCCACCCTTACGGTTGCGCTCAATGCTGAACTTAAAAAACATGCAGCAACACGTTGGCGTGAAGAGAACG
CAGAAGCTATCGCTGCGTTAAATCAATTGGCTGATGAAACCGGATGTTTCTCTGATGAGTACCGGAGCTTCTAG