Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | vapBC/LOTUS_5_Limkain_b1-Phd |
| Location | 989474..990165 | Replicon | plasmid p1 |
| Accession | NZ_CP071400 | ||
| Organism | Rhizobium leguminosarum strain OyaliB | ||
Toxin (Protein)
| Gene name | vapC | Uniprot ID | - |
| Locus tag | J0664_RS28410 | Protein ID | WP_207159047.1 |
| Coordinates | 989737..990165 (+) | Length | 143 a.a. |
Antitoxin (Protein)
| Gene name | vapB | Uniprot ID | A0A4R0AD13 |
| Locus tag | J0664_RS28405 | Protein ID | WP_017991284.1 |
| Coordinates | 989474..989740 (+) | Length | 89 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J0664_RS28370 | 984671..984928 | + | 258 | WP_003550229.1 | hypothetical protein | - |
| J0664_RS28375 | 984928..985299 | + | 372 | WP_207159042.1 | type II toxin-antitoxin system RelE/ParE family toxin | - |
| J0664_RS28380 | 985571..986296 | - | 726 | WP_207159043.1 | ATP-binding cassette domain-containing protein | - |
| J0664_RS28385 | 986274..986933 | - | 660 | WP_017956570.1 | amino acid ABC transporter permease | - |
| J0664_RS28390 | 986930..987601 | - | 672 | WP_207159044.1 | ABC transporter permease subunit | - |
| J0664_RS28395 | 987681..988466 | - | 786 | WP_207159045.1 | transporter substrate-binding domain-containing protein | - |
| J0664_RS28400 | 988470..989219 | - | 750 | WP_207159046.1 | GntR family transcriptional regulator | - |
| J0664_RS28405 | 989474..989740 | + | 267 | WP_017991284.1 | type II toxin-antitoxin system Phd/YefM family antitoxin | Antitoxin |
| J0664_RS28410 | 989737..990165 | + | 429 | WP_207159047.1 | PIN domain-containing protein | Toxin |
| J0664_RS28415 | 990206..990976 | - | 771 | WP_207159048.1 | ATP-binding cassette domain-containing protein | - |
| J0664_RS28420 | 990989..991648 | - | 660 | WP_024319797.1 | amino acid ABC transporter permease | - |
| J0664_RS28425 | 991653..992315 | - | 663 | WP_207159049.1 | ABC transporter permease subunit | - |
| J0664_RS28430 | 992429..993268 | - | 840 | WP_017956578.1 | transporter substrate-binding domain-containing protein | - |
| J0664_RS28435 | 993431..993646 | - | 216 | Protein_899 | PAS domain-containing protein | - |
| J0664_RS28440 | 993633..993961 | - | 329 | Protein_900 | flagellar protein | - |
| J0664_RS28445 | 993967..994614 | - | 648 | WP_168342365.1 | TetR/AcrR family transcriptional regulator | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Conjugative plasmid | - | - | 1..1230906 | 1230906 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 143 a.a. Molecular weight: 15819.14 Da Isoelectric Point: 4.5593
>T196315 WP_207159047.1 NZ_CP071400:989737-990165 [Rhizobium leguminosarum]
VRLLLDTNVLSEVTRPCPDARVLEWLDSLDEDRSFISTVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVTIAWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGLGIELVDPWIERP
VRLLLDTNVLSEVTRPCPDARVLEWLDSLDEDRSFISTVSIAEIRRGVALMESGRKRDALAEWLAQDLPQRFEQRVIPVD
QPVTIAWGDLMGLAKRSGRGLSSMDGLIAATAIAHDLTLATRNIKDFEGLGIELVDPWIERP
Download Length: 429 bp
>T196315 NZ_CP095086:c3894928-3894551 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCACCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGAAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA
ATGAAAACATTACCTGTATTACCCGGGCTGGCGGCCAGTTCTCACCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGAAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA
Antitoxin
Download Length: 89 a.a. Molecular weight: 9960.34 Da Isoelectric Point: 10.0842
>AT196315 WP_017991284.1 NZ_CP071400:989474-989740 [Rhizobium leguminosarum]
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL
MQPSRPEDKSWTVASAKAKLSEVIERAQATPQTIMRNGKPSVVVVSAEEWQRKTARKGTLAEFLMQSPLRGADLDLERQR
DEPRDLQL
Download Length: 267 bp
>AT196315 NZ_CP095086:c3895385-3895017 [Escherichia coli]
GTGTCAGACATACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
GTGTCAGACATACTGCCCGGGACAACGCATCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|