Detailed information of TA system TA196314

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): tacAT/DUF1778(antitoxin)
• Location: 846795..847597
• Replicon: plasmid p1
• Accession: NZ_CP071400
• Organism: Rhizobium leguminosarum strain OyaliB

Toxin (Protein)

• Gene name: TacT3
• Uniprot ID: -
• Locus tag: J0664_RS27730
• Protein ID: WP_207158959.1
• Coordinates: 847070..847597 (+)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: TacA3
• Uniprot ID: A0A1B1CH27
• Locus tag: J0664_RS27725
• Protein ID: WP_020046930.1
• Coordinates: 846795..847073 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0664_RS27705	841970..843487	-	1518	WP_207158956.1	ABC transporter substrate-binding protein	-
J0664_RS27710	843702..844487	+	786	WP_207159456.1	helix-turn-helix domain-containing protein	-
J0664_RS27715	844558..845280	+	723	WP_207158957.1	ribonuclease activity regulator RraA	-
J0664_RS27720	845695..846573	+	879	WP_207158958.1	exopolysaccharide synthesis protein	-
J0664_RS27725	846795..847073	+	279	WP_020046930.1	DUF1778 domain-containing protein	Antitoxin
J0664_RS27730	847070..847597	+	528	WP_207158959.1	GNAT family N-acetyltransferase	Toxin
J0664_RS27735	847892..849535	+	1644	WP_207158960.1	acetolactate synthase large subunit	-
J0664_RS27740	849886..851010	+	1125	WP_207158961.1	metallophosphoesterase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1230906	1230906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Vibrio phage ICP1, Orf50 (12-87)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 18747.69 Da
• Isoelectric Point: 9.6306

>T196314 WP_207158959.1 NZ_CP071400:847070-847597 [Rhizobium leguminosarum]
VTLPVWHEEPIAKSHDRASFDCGDGAMNEFIRRFARQSHEQNAAKTFCAIDKAQPDRILGFYTVAPSAVTHEAVPPKLTR
GLARHEVAGFKLARLATDIKVAGKGLGGQLIAAAALRCLRLASEGGGILLIIDAKSERAAHWHASYGAEPLQGKPLTLVM
PLATFAADFKAKGLL

Nucleotide

• Length: 528 bp

>T196314 NZ_CP095086:3695433-3695651 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10189.87 Da
• Isoelectric Point: 8.6184

>AT196314 WP_020046930.1 NZ_CP071400:846795-847073 [Rhizobium leguminosarum]
MPRTADNKTERYQLRIPPKQKAMIARAAALSNKDMADFILDKIVPEAEAVIQAAEVETVSSRDFIRILDLLEHPPKPNAK
LRAAISALPDAL

Nucleotide

• Length: 279 bp

>AT196314 NZ_CP095086:3695033-3695407 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTTAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Antitoxin

• AlphaFold DB: A0A1B1CH27