Detailed information of TA system TA196313

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-StbC
• Location: 776530..777206
• Replicon: plasmid p1
• Accession: NZ_CP071400
• Organism: Rhizobium leguminosarum strain OyaliB

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J0664_RS27415
• Protein ID: WP_131661221.1
• Coordinates: 776781..777206 (+)
• Length: 142 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A1S9GB48
• Locus tag: J0664_RS27410
• Protein ID: WP_065282799.1
• Coordinates: 776530..776784 (+)
• Length: 85 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0664_RS27395	772094..773692	-	1599	WP_077980611.1	ABC transporter substrate-binding protein	-
J0664_RS27400	773676..774419	-	744	WP_168255521.1	FadR family transcriptional regulator	-
J0664_RS27405	774595..775812	-	1218	WP_207158923.1	diguanylate cyclase	-
J0664_RS27410	776530..776784	+	255	WP_065282799.1	plasmid stabilization protein	Antitoxin
J0664_RS27415	776781..777206	+	426	WP_131661221.1	type II toxin-antitoxin system VapC family toxin	Toxin
J0664_RS27420	777506..779677	+	2172	WP_168259570.1	TonB-dependent siderophore receptor	-
J0664_RS27425	779682..780470	+	789	WP_077980616.1	siderophore-iron reductase FhuF	-
J0664_RS27430	780552..781043	-	492	WP_077980617.1	GNAT family N-acetyltransferase	-
J0664_RS27435	781207..781866	+	660	WP_168255528.1	sigma-70 family RNA polymerase sigma factor	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1230906	1230906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (2-124)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 142 a.a.
• Molecular weight: 15325.61 Da
• Isoelectric Point: 4.8011

>T196313 WP_131661221.1 NZ_CP071400:776781-777206 [Rhizobium leguminosarum]
MILIDTNVISEPWKPTPDYQVLAWIDAQAVETLFVSAITVAELRFGIAAMPSGRRKTVLYDRLEQEVLPLFSGRVLSFDM
AASQSYSELMARARLAGRAIGKADAYIAATAAANGFIVATRDTSPFEAAGLNVINPWYAPT

Nucleotide

• Length: 426 bp

>T196313 NZ_CP095086:c3004777-3004397 [Escherichia coli]
ATGAAAACATTACCTGACACACACGTACGGGAGGCATCGCGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGTTGGACCAGCATTACGGCCTCACACTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTTGTTGAAAAATACGCGCTGGTACGAACTGACCAGCCGGGATTC
AGCGCAGGAGTCCCGTCGCAGTTAATTAACAGCATCGATATTCTCCGGGCCCGCCGGGCAACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATGACATTACCCTGGGCAGGCATCCGGAGGAGGCGAAACAATGA

Antitoxin

Protein

• Length: 85 a.a.
• Molecular weight: 9192.38 Da
• Isoelectric Point: 6.2221

>AT196313 WP_065282799.1 NZ_CP071400:776530-776784 [Rhizobium leguminosarum]
MAAVTIRNLSDETHRALRVRAAHHGRSTEAEIRDILETAVRPAERVMLGSLLASIGREAGLSNNDVDNLQLVRDKAAAEP
MSFE

Nucleotide

• Length: 255 bp

>AT196313 NZ_CP095086:c3005198-3004824 [Escherichia coli]
GTGTCAGACACGCTCCATGAAACAAATTATCCCGACGACAACAACGACCGCCCCTGGTGGGGGCTACCTTGCACCGTGAC
GCCCTGTTTCGGGGCACGTCTGGTGCAGGAGGGCAATCAGTTGCATTACCTTGCCGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCATACCATCTGGACCAGGCTTTTCCACTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCCAGAGGGCTGACCTGCGAAGCCGACACCCTCGGCAGCTG
TGGTTACGTTTATATGGCTGTTTATCCGACACTGGCACCCGCAACCACCTCATAA

Structures

Antitoxin

• AlphaFold DB: A0A1S9GB48