Detailed information of TA system TA196311

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/PIN(toxin)
• Location: 254248..254915
• Replicon: plasmid p1
• Accession: NZ_CP071400
• Organism: Rhizobium leguminosarum strain OyaliB

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: J0BF41
• Locus tag: J0664_RS25095
• Protein ID: WP_003551326.1
• Coordinates: 254472..254915 (+)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J0664_RS25090
• Protein ID: WP_003555338.1
• Coordinates: 254248..254475 (+)
• Length: 76 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0664_RS25070	250621..251163	-	543	WP_207159238.1	GNAT family N-acetyltransferase	-
J0664_RS25075	251163..251471	-	309	WP_065283387.1	DUF1778 domain-containing protein	-
J0664_RS25080	252839..253453	-	615	WP_207159239.1	LysE family transporter	-
J0664_RS25085	253775..253978	+	204	WP_077978140.1	cold-shock protein	-
J0664_RS25090	254248..254475	+	228	WP_003555338.1	hypothetical protein	Antitoxin
J0664_RS25095	254472..254915	+	444	WP_003551326.1	PIN domain-containing protein	Toxin
J0664_RS25100	255507..257036	-	1530	WP_168344073.1	ABC transporter substrate-binding protein	-
J0664_RS25105	257064..257891	-	828	WP_207159240.1	ABC transporter permease subunit	-
J0664_RS25110	257888..258859	-	972	WP_168344075.1	ABC transporter permease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..1230906	1230906

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain toxin (2-131)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 15595.83 Da
• Isoelectric Point: 7.4219

>T196311 WP_003551326.1 NZ_CP071400:254472-254915 [Rhizobium leguminosarum]
VTFLLDVNVLIALIDPGHVAHDDAHEWFAAIGQSAWATCPITENGVIRIVGNPKYPNSPGSPSLVMEIVRKLRSLPGHSF
WPDDVSLVGSGDIAPTKILTSGQVTDTYLLALAKARGGQLATFDRKLSAAAVTRGNSALHLITTSRS

Nucleotide

• Length: 444 bp

>T196311 NZ_CP095086:2574611-2574889 [Escherichia coli]
ATGATAATGAATTTCAGACACAAGGGATTGCGTGACTTGTTTCTTCTCGGCAAAACCTCCGGCGTTATACCGACGCAAGT
CAAACGATTACGCCACCGACTCGCTGTGATTGATGCAGCATGCTGTCTTGCTGATATCGATATGCCCGGTTACCGACTAC
ATCCGTTAAGCGGCGATCGCGATGGAATTTGGGCGATATCTGTCTCGGGCAACTGGCGAATCACATTTGAATTCGTCAAT
GGCGATGCATACATACTGGATTACGAGGACTATCACTGA

Antitoxin

Protein

• Length: 76 a.a.
• Molecular weight: 8220.39 Da
• Isoelectric Point: 7.0120

>AT196311 WP_003555338.1 NZ_CP071400:254248-254475 [Rhizobium leguminosarum]
MRTTLAIDDDVLTAAKAMATQQHRSVGEVISELARRSLRRPPSSGERNGIPLLSTRPDTPPVTLEIVNAMRDELP

Nucleotide

• Length: 228 bp

>AT196311 NZ_CP095086:2574889-2575173 [Escherichia coli]
ATGAAAATGGCCAATCATCCCCGCCCGGGGGACATTATTCAGGAATCACTGGACGAACTTAATGTCAGCCTGCGCGAGTT
TGCCAGAGCAATGGAAATTGCGCCCTCAACGGCAAGCCGATTGCTGACCGGAAAAGCAGCTTTGACGCCAGAAATGGCAA
TAAAACTTTCCGTGGTGATCGGCAGTTCGCCGCAAATGTGGCTGAATCTGCAAAATGCCTGGAGTCTGGCAGAGGCGGAA
AAAACGGTGGATGTGTCGCGACTGCGCCGTCTGGTTACGCAATAA

Structures

Toxin

• AlphaFold DB: J0BF41