Detailed information of TA system TA196308

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/VapC-VagC
• Location: 1129768..1130441
• Replicon: chromosome
• Accession: NZ_CP071399
• Organism: Rhizobium leguminosarum strain OyaliB

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J0664_RS05280
• Protein ID: WP_207157807.1
• Coordinates: 1130028..1130441 (+)
• Length: 138 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: -
• Locus tag: J0664_RS05275
• Protein ID: WP_207157806.1
• Coordinates: 1129768..1130031 (+)
• Length: 88 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0664_RS05260	1124867..1126810	+	1944	WP_027664285.1	PrkA family serine protein kinase	-
J0664_RS05265	1126823..1128097	+	1275	WP_065280660.1	YeaH/YhbH family protein	-
J0664_RS05270	1128094..1129644	+	1551	WP_065280659.1	SpoVR family protein	-
J0664_RS05275	1129768..1130031	+	264	WP_207157806.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J0664_RS05280	1130028..1130441	+	414	WP_207157807.1	PIN domain-containing protein	Toxin
J0664_RS05285	1130621..1131535	+	915	WP_207157808.1	adenylate/guanylate cyclase domain-containing protein	-
J0664_RS05290	1131804..1133012	+	1209	WP_207157809.1	efflux RND transporter periplasmic adaptor subunit	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (5-124)

Antitoxin

• SpoVT-AbrB domain (9-54)

Sequences

Toxin

Protein

• Length: 138 a.a.
• Molecular weight: 15154.50 Da
• Isoelectric Point: 5.6193

>T196308 WP_207157807.1 NZ_CP071399:1130028-1130441 [Rhizobium leguminosarum]
MTAWMLDTNMVSHVIKGDRPEIIERLAALPIEEIVISSITEGELFYGLAKRGYPKALSERIRQFLLRVDVLPWDHDVTRT
HGDLRAACDAKGVTLAPLDMMIAAHAVATAATLVTRDKAFARVSEPLKVDDWANEGS

Nucleotide

• Length: 414 bp

>T196308 NZ_CP095086:c1964158-1963784 [Escherichia coli]
ATGAAAACATTACCTGTATTACCCGGGCAGGCGGCCAGTTCTCGCCCGTCTCCTGTTGAAATCTGGCAGATACTGCTGTC
CCGACTGCTGGACCAGCACTACGGCCTCACACTGAATGACACACCGTTTGCCGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGCATTTCACTGTGTGATGCGGTGAACTTTCTCGTGGAAAAATACGCGCTGGTGCGTACCGACCAGCCGGGATTC
AGCGCCTGTACCCGCTCTCAGTTAATAAACAGCATCGATATCCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGCGA
CAATTACAGAACGGTAAATAACATTACCCTGGGTAAGTATCCGGAGGCGAAATGA

Antitoxin

Protein

• Length: 88 a.a.
• Molecular weight: 10184.36 Da
• Isoelectric Point: 7.0174

>AT196308 WP_207157806.1 NZ_CP071399:1129768-1130031 [Rhizobium leguminosarum]
MAKRTAKIFTTGRSQAVRLPAEFRFEESEVFVRRDPKSGDVILSRKPNSWDGLFELYGKDRVPDDFLGPDDRRQPSHDRD
PFEGWKE

Nucleotide

• Length: 264 bp

>AT196308 NZ_CP095086:c1964615-1964247 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACACTTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTACCCTGCACCGTGAC
GCCCTGTTTCGGCGCCCGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCCGACCGTGCCGGTATCAGAGGCCTGT
TCAGCGATGCAGATGCGTACCACCTGGACCAGGCCTTTCCGTTGCTGATGAAACAACTGGAGCTCATGCTCACCAGTGGT
GAACTCAGCCCCCGCTATCAGCATACCGTCACGCTGTATGCGAAAGGACTGACCTGCGAAGCCGATACCCTGGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACGCCCGAAATGAAAAATTAA