Detailed information of TA system TA196307

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): stbDE/ParE-YafN
• Location: 143879..144401
• Replicon: plasmid pKmfe267-2
• Accession: NZ_CP071395
• Organism: Klebsiella michiganensis strain Kmfe267

Toxin (Protein)

• Gene name: stbE
• Uniprot ID: -
• Locus tag: J1F62_RS29855
• Protein ID: WP_207172923.1
• Coordinates: 144117..144401 (+)
• Length: 95 a.a.

Antitoxin (Protein)

• Gene name: stbD
• Uniprot ID: A0A376C2P3
• Locus tag: J1F62_RS29850
• Protein ID: WP_048030981.1
• Coordinates: 143879..144127 (+)
• Length: 83 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F62_RS29825	140269..141078	-	810	WP_207172921.1	hypothetical protein	-
J1F62_RS29830	141359..141787	-	429	WP_001554382.1	GFA family protein	-
J1F62_RS29835	141965..142567	+	603	WP_207172922.1	tyrosine-type recombinase/integrase	-
J1F62_RS29840	142784..143020	+	237	WP_139316165.1	hypothetical protein	-
J1F62_RS29845	143161..143460	-	300	WP_075912736.1	antibiotic biosynthesis monooxygenase	-
J1F62_RS29850	143879..144127	+	249	WP_048030981.1	plasmid stabilization protein	Antitoxin
J1F62_RS29855	144117..144401	+	285	WP_207172923.1	type II toxin-antitoxin system mRNA interferase toxin, RelE/StbE family	Toxin
J1F62_RS29860	144417..145741	-	1325	Protein_160	ISNCY family transposase	-
J1F62_RS29865	145909..146232	-	324	WP_195729748.1	theronine dehydrogenase	-
J1F62_RS29870	146229..146957	-	729	WP_207172924.1	plasmid SOS inhibition protein A	-
J1F62_RS29875	146954..147385	-	432	WP_075912739.1	conjugation system SOS inhibitor PsiB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..170909	170909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-84)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 95 a.a.
• Molecular weight: 10960.69 Da
• Isoelectric Point: 10.4237

>T196307 WP_207172923.1 NZ_CP071395:144117-144401 [Klebsiella michiganensis]
MTYKLAFNESALKEWKKLGHTLQVQFKKKLKERLENPRVPASQLHGRKDQYKIKLLGAGYRLVYRVEDDIVTVTVIGVGK
RENDDIYNATLNRN

Nucleotide

• Length: 285 bp

>T196307 NZ_CP095086:1945032-1945286 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Protein

• Length: 83 a.a.
• Molecular weight: 9039.25 Da
• Isoelectric Point: 3.9947

>AT196307 WP_048030981.1 NZ_CP071395:143879-144127 [Klebsiella michiganensis]
MAFQVLTTTAASITELKRDPMGTFHAGDGAPVAILNRNEPAFYCVPPALYAQLMELLEDEELGRMADERSGERVIEVNID
DL

Nucleotide

• Length: 249 bp

>AT196307 NZ_CP095086:1944784-1945035 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Structures

Antitoxin

• AlphaFold DB: A0A376C2P3