Detailed information of TA system TA196306

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 80938..81581
• Replicon: plasmid pKmfe267-2
• Accession: NZ_CP071395
• Organism: Klebsiella michiganensis strain Kmfe267

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: -
• Locus tag: J1F62_RS29495
• Protein ID: WP_207172963.1
• Coordinates: 81165..81581 (+)
• Length: 139 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q84A07
• Locus tag: J1F62_RS29490
• Protein ID: WP_001261276.1
• Coordinates: 80938..81168 (+)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F62_RS29460	75949..76800	+	852	WP_040217077.1	MurR/RpiR family transcriptional regulator	-
J1F62_RS29465	76855..77604	+	750	WP_059359287.1	N-formylglutamate amidohydrolase	-
J1F62_RS29470	77608..78255	+	648	WP_075912792.1	cysteine hydrolase	-
J1F62_RS29475	78531..78803	-	273	Protein_83	Tn3 family transposase	-
J1F62_RS29480	79251..80012	+	762	WP_207172961.1	hypothetical protein	-
J1F62_RS29485	80048..80686	-	639	WP_207172962.1	hypothetical protein	-
J1F62_RS29490	80938..81168	+	231	WP_001261276.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J1F62_RS29495	81165..81581	+	417	WP_207172963.1	PIN domain-containing protein	Toxin
J1F62_RS29500	81713..81829	+	117	Protein_88	transposase	-
J1F62_RS29505	82049..82363	-	315	Protein_89	IS110 family transposase	-
J1F62_RS29510	83117..83320	+	204	WP_207172964.1	hemolysin expression modulator Hha	-
J1F62_RS29515	83426..83620	-	195	WP_207172965.1	hypothetical protein	-
J1F62_RS29520	84119..85519	+	1401	WP_207172966.1	hypothetical protein	-
J1F62_RS29525	85533..85919	+	387	WP_207172858.1	hypothetical protein	-
J1F62_RS29530	86142..86492	-	351	WP_207172859.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..170909	170909
	-	inside	IScluster/Tn	-	-	78531..82282	3751

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 139 a.a.
• Molecular weight: 15064.57 Da
• Isoelectric Point: 7.2453

>T196306 WP_207172963.1 NZ_CP071395:81165-81581 [Klebsiella michiganensis]
MKKTWMLDTNICSFIMREQLAAVLMRLEQAVLHGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAADAILVTNNVREFGRVPGLVLEDWVK

Nucleotide

• Length: 417 bp

>T196306 NZ_CP095086:1944784-1945035 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCCCTGGAGGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCTGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8721.74 Da
• Isoelectric Point: 4.3533

>AT196306 WP_001261276.1 NZ_CP071395:80938-81168 [Klebsiella michiganensis]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRADPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT196306 NZ_CP095086:1945032-1945286 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCAGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Antitoxin

• AlphaFold DB: A0A387K023