Detailed information of TA system TA196305

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ccdAB/CcdA(antitoxin)
• Location: 35133..35658
• Replicon: plasmid pKmfe267-2
• Accession: NZ_CP071395
• Organism: Klebsiella michiganensis strain Kmfe267

Toxin (Protein)

• Gene name: ccdB
• Uniprot ID: -
• Locus tag: J1F62_RS29235
• Protein ID: WP_207172950.1
• Coordinates: 35133..35438 (-)
• Length: 102 a.a.

Antitoxin (Protein)

• Gene name: ccdA
• Uniprot ID: J6I6S5
• Locus tag: J1F62_RS29240
• Protein ID: WP_009653145.1
• Coordinates: 35440..35658 (-)
• Length: 73 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F62_RS29205	31229..31527	-	299	Protein_29	transposase	-
J1F62_RS29210	31825..32187	-	363	WP_100781538.1	protease inhibitor I42 family protein	-
J1F62_RS29215	32730..33443	-	714	Protein_31	HNH endonuclease	-
J1F62_RS29220	33811..34248	+	438	WP_207172949.1	VOC family protein	-
J1F62_RS29225	34414..34569	-	156	Protein_33	chromosome partitioning protein ParB	-
J1F62_RS29230	34582..34933	-	352	Protein_34	hypothetical protein	-
J1F62_RS29235	35133..35438	-	306	WP_207172950.1	type II toxin-antitoxin system toxin CcdB	Toxin
J1F62_RS29240	35440..35658	-	219	WP_009653145.1	type II toxin-antitoxin system antitoxin CcdA	Antitoxin
J1F62_RS29245	36060..36227	+	168	Protein_37	CAAX protease	-
J1F62_RS29250	36313..37548	-	1236	WP_207172951.1	MFS transporter	-
J1F62_RS29255	37558..39267	-	1710	WP_207172971.1	hypothetical protein	-
J1F62_RS29260	40159..40587	+	429	WP_047718108.1	TOBE domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	-	-	1..170909	170909

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin CcdB (2-100)

Antitoxin

• Post-segregation antitoxin CcdA (2-72)

Sequences

Toxin

Protein

• Length: 102 a.a.
• Molecular weight: 11641.38 Da
• Isoelectric Point: 6.4661

>T196305 WP_207172950.1 NZ_CP071395:c35438-35133 [Klebsiella michiganensis]
MQFKVYAYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDKVSRELYPVVHIGDDSYRLMTTDMASVTALVTGEEV
TDLSHRENDIKNAINLMFWGI

Nucleotide

• Length: 306 bp

>T196305 NZ_CP095086:1213849-1214160 [Escherichia coli]
ATGCACATCATTTCTAAAGCGCCATTTGAAGAGTCTGCGCGTAAATATCCTAACGACGCTCTGGCTCTTCAGGCATTGTA
TCGGGTTATTAAGGAAACGGATTTTTCTACGCCAGAAGAAATGCGAACGGCGTTTCCCAACCTCGACAATTTTAAATATC
GCAACAAATGGTACGTTCTGGATGTAGGTGGGAACAACCTAAGGGTGATTGCCTACATAAACTTTGTTAATAAGCGGTTC
TTTGTGAAGCACATAACTAATCACGCCGAGTATGACAAACTGACACGTTACTACAGAGAGAACAAAGAATGA

Antitoxin

Protein

• Length: 73 a.a.
• Molecular weight: 8318.25 Da
• Isoelectric Point: 4.5045

>AT196305 WP_009653145.1 NZ_CP071395:c35658-35440 [Klebsiella michiganensis]
MKQRITVTIDSDNYQLLKAADINISGLVNTAMQNEARRLRAERWQAENQEGMAEVARFIDMNGSFADENRDW

Nucleotide

• Length: 219 bp

>AT196305 NZ_CP095086:1214157-1214576 [Escherichia coli]
ATGACTGCTAATGCTGTACGCGCTGTTAAAGCAACAAGAGAACTGGTCAATGCAGTCCCCTTTCTGGGAGGCAGTGACTC
AGAAGATGATTATCGTGAAGCACTGGAACTGGTCGAATATTTGATTGAAGAAGATGACACCAATCCACTTATCGACTTTC
TGACGAGCCGCATTGCAGAATATGAAAACAACAACGAAAAGTTTGCAGAGTTCGACAAAGCCGTTGCGGCAATGCCTGTT
GGTGTGGCGTTACTTCGCACGCTGATTGATCAACATAATCTGACCTATGCGGATTTGAAGAACGAAATCGGCTCTAAATC
CCTGGTCAGCCAGATCTTGTCTGGACAACGATCCCTGACGATATCCCACATTAAGGCACTTTCAGCCCGATTTGGCGTTA
AGCCGGAATGGTTTCTTTGA

Structures

Antitoxin

• AlphaFold DB: J6I6S5