196304

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) stbDE/ParE-YafN
Location 174529..175051 Replicon plasmid pKmfe267-1
Accession NZ_CP071394
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name stbE Uniprot ID A0A2J4R0S6
Locus tag J1F62_RS28705 Protein ID WP_004181778.1
Coordinates 174767..175051 (+) Length 95 a.a.

Antitoxin (Protein)


Gene name stbD Uniprot ID A0A2J4R0U8
Locus tag J1F62_RS28700 Protein ID WP_004181777.1
Coordinates 174529..174777 (+) Length 83 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS28680 169738..170559 - 822 WP_004181772.1 hypothetical protein -
J1F62_RS28685 170621..170974 - 354 WP_004181774.1 hypothetical protein -
J1F62_RS28690 171119..172105 - 987 WP_094965947.1 hypothetical protein -
J1F62_RS28695 172439..174238 - 1800 WP_004181776.1 ATP-dependent helicase -
J1F62_RS28700 174529..174777 + 249 WP_004181777.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
J1F62_RS28705 174767..175051 + 285 WP_004181778.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
J1F62_RS28710 175068..175169 - 102 Protein_187 IS200/IS605 family transposase -
J1F62_RS28715 175205..176431 + 1227 WP_040209639.1 transposase -
J1F62_RS28720 176702..176926 - 225 Protein_189 transposase -
J1F62_RS28725 177005..177424 - 420 WP_100757109.1 IS200/IS605 family transposase -
J1F62_RS28730 177460..178647 + 1188 WP_040209642.1 transposase -
J1F62_RS28735 178746..179841 - 1096 Protein_192 conjugal transfer protein TraG N-terminal domain-containing protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / qnrB4 / blaDHA-1 / blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / catA2 / mph(A) - 1..251953 251953


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, RelE/ParE toxin family

(6-84)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 95 a.a.        Molecular weight: 10970.69 Da        Isoelectric Point: 10.6516

>T196304 WP_004181778.1 NZ_CP071394:174767-175051 [Klebsiella michiganensis]
MTYKLAFNESALKEWKKLGHTIREQFKKKLAERLLNPRVPASQLHGRKDQYKIKLRGAGYRLVYSVNDDVVTVTVIGVGK
RENDDIYNLTKHRN

Download         Length: 285 bp

>T196304 NZ_CP095086:980969-981376 [Escherichia coli]
GTGGTCCTGTGGCAATCTGATTTGCGCGTCTCCTGGCGCGCGCAGTGGCTTTCCTTGCTGATCCACGGGCTGGTTGCCGC
TGTTATTTTACTCATGCCCTGGCCGCTCAGTTACACCCCGTTATGGATGGTGTTACTTTCGCTGGTGGTGTTTGATTGCG
TTCGCAGCCAGCGACGCATTAATGCTCGCCAGGGGGAAATTCTCCTGTTGATGGACGGGCGTTTGCGTTGGCAAGGGCAG
GAGTGGAGCATCGTCAAAGCGCCGTGGATGATTAAGAGCGGCATGATGCTGCGTTTACGTTCTGACAGCGGAAAACGGCA
ACATTTATGGCTGGCAGCTGACAGCATGGACGAAGCCGAATGGCGGGATTTACGGCGGATATTGTTACAACAAGAGACGC
AAAGATAA

Antitoxin


Download         Length: 83 a.a.        Molecular weight: 8925.17 Da        Isoelectric Point: 3.8209

>AT196304 WP_004181777.1 NZ_CP071394:174529-174777 [Klebsiella michiganensis]
MAFQVLTTTAASITELKRDPMGTFNAGEGAPVAILNRNEPAFYCVPPALYAQLMELLEDQELGRIADERIGEAVIEVSLD
DL

Download         Length: 249 bp

>AT196304 NZ_CP095086:980722-980988 [Escherichia coli]
ATGGACATTAACAACAAAGCCCGCATTCATTGGGCATGCCGCCGTGGTATGCGCGAACTCGATATTTCAATCATGCCGTT
TTTCGAACATGAGTACGACAGCTTAAGCGATGACGAAAAACGCATCTTTATTCGTCTGCTGGAATGTGACGATCCGGACC
TGTTTAACTGGCTGATGAATCACGGTAAACCGGCCGATGCAGAACTGGAAATGATGGTCCGACTCATCCAGACACGGAAC
CGGGAACGTGGTCCTGTGGCAATCTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J4R0S6

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4R0U8

References