Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ataRT/ElaA-DUF1778
Location 82406..83157 Replicon plasmid pKmfe267-1
Accession NZ_CP071394
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name ataT Uniprot ID H6U1U8
Locus tag J1F62_RS28215 Protein ID WP_014386536.1
Coordinates 82675..83157 (+) Length 161 a.a.

Antitoxin (Protein)


Gene name ataR Uniprot ID A0A071LPN3
Locus tag J1F62_RS28210 Protein ID WP_004902250.1
Coordinates 82406..82684 (+) Length 93 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS28175 78049..78474 - 426 WP_000522996.1 organomercurial transporter MerC -
J1F62_RS28180 78502..78777 - 276 WP_000732275.1 mercury resistance system periplasmic binding protein MerP -
J1F62_RS28185 78793..79158 - 366 WP_001294656.1 mercuric ion transporter MerT -
J1F62_RS28190 79230..79685 + 456 WP_001166628.1 Hg(II)-responsive transcriptional regulator -
J1F62_RS28195 80338..80796 + 459 WP_014386535.1 hypothetical protein -
J1F62_RS28200 81626..81967 + 342 WP_004902257.1 hypothetical protein -
J1F62_RS28205 82075..82287 + 213 WP_004902255.1 hypothetical protein -
J1F62_RS28210 82406..82684 + 279 WP_004902250.1 DUF1778 domain-containing protein Antitoxin
J1F62_RS28215 82675..83157 + 483 WP_014386536.1 GNAT family N-acetyltransferase Toxin
J1F62_RS28220 83958..84926 + 969 WP_074443085.1 IS5 family transposase -
J1F62_RS28225 85258..85797 + 540 WP_004902239.1 hypothetical protein -
J1F62_RS28230 85902..86294 + 393 WP_032442757.1 hypothetical protein -
J1F62_RS28235 86395..87150 + 756 WP_004902235.1 DUF2971 domain-containing protein -
J1F62_RS28240 87177..87824 + 648 WP_014386537.1 EcsC family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / qnrB4 / blaDHA-1 / blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / catA2 / mph(A) - 1..251953 251953
- flank IS/Tn - - 83958..84926 968


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(42-144)

Antitoxin

Vibrio phage ICP1, Orf50

(10-86)


Sequences

Toxin        


Download         Length: 161 a.a.        Molecular weight: 17662.45 Da        Isoelectric Point: 8.9130

>T196303 WP_014386536.1 NZ_CP071394:82675-83157 [Klebsiella michiganensis]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV

Download         Length: 483 bp

>T196303 NZ_CP095086:c821625-821248 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATGCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin


Download         Length: 93 a.a.        Molecular weight: 10704.23 Da        Isoelectric Point: 4.5271

>AT196303 WP_004902250.1 NZ_CP071394:82406-82684 [Klebsiella michiganensis]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE

Download         Length: 279 bp

>AT196303 NZ_CP095086:c822083-821715 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCTGACGACAATCACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2A5MBI1

Antitoxin

Source ID Structure
AlphaFold DB A0A071LPN3

References