Detailed information of TA system
Overview
TA module
| Type | II | Classification (family/domain) | ataRT/ElaA-DUF1778 |
| Location | 82406..83157 | Replicon | plasmid pKmfe267-1 |
| Accession | NZ_CP071394 | ||
| Organism | Klebsiella michiganensis strain Kmfe267 | ||
Toxin (Protein)
| Gene name | ataT | Uniprot ID | H6U1U8 |
| Locus tag | J1F62_RS28215 | Protein ID | WP_014386536.1 |
| Coordinates | 82675..83157 (+) | Length | 161 a.a. |
Antitoxin (Protein)
| Gene name | ataR | Uniprot ID | A0A071LPN3 |
| Locus tag | J1F62_RS28210 | Protein ID | WP_004902250.1 |
| Coordinates | 82406..82684 (+) | Length | 93 a.a. |
Genomic Context
| Locus tag | Coordinates | Strand | Size (bp) | Protein ID | Product | Description |
|---|---|---|---|---|---|---|
| J1F62_RS28175 | 78049..78474 | - | 426 | WP_000522996.1 | organomercurial transporter MerC | - |
| J1F62_RS28180 | 78502..78777 | - | 276 | WP_000732275.1 | mercury resistance system periplasmic binding protein MerP | - |
| J1F62_RS28185 | 78793..79158 | - | 366 | WP_001294656.1 | mercuric ion transporter MerT | - |
| J1F62_RS28190 | 79230..79685 | + | 456 | WP_001166628.1 | Hg(II)-responsive transcriptional regulator | - |
| J1F62_RS28195 | 80338..80796 | + | 459 | WP_014386535.1 | hypothetical protein | - |
| J1F62_RS28200 | 81626..81967 | + | 342 | WP_004902257.1 | hypothetical protein | - |
| J1F62_RS28205 | 82075..82287 | + | 213 | WP_004902255.1 | hypothetical protein | - |
| J1F62_RS28210 | 82406..82684 | + | 279 | WP_004902250.1 | DUF1778 domain-containing protein | Antitoxin |
| J1F62_RS28215 | 82675..83157 | + | 483 | WP_014386536.1 | GNAT family N-acetyltransferase | Toxin |
| J1F62_RS28220 | 83958..84926 | + | 969 | WP_074443085.1 | IS5 family transposase | - |
| J1F62_RS28225 | 85258..85797 | + | 540 | WP_004902239.1 | hypothetical protein | - |
| J1F62_RS28230 | 85902..86294 | + | 393 | WP_032442757.1 | hypothetical protein | - |
| J1F62_RS28235 | 86395..87150 | + | 756 | WP_004902235.1 | DUF2971 domain-containing protein | - |
| J1F62_RS28240 | 87177..87824 | + | 648 | WP_014386537.1 | EcsC family protein | - |
Associated MGEs
| MGE detail |
Similar MGEs |
Relative position |
MGE Type | Cargo ARG | Virulence gene | Coordinates | Length (bp) |
|---|---|---|---|---|---|---|---|
| - | inside | Non-Mobilizable plasmid | tet(A) / dfrA12 / aadA2 / qacE / sul1 / qnrB4 / blaDHA-1 / blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / catA2 / mph(A) | - | 1..251953 | 251953 | |
| - | flank | IS/Tn | - | - | 83958..84926 | 968 |
Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.
Sequences
Toxin
Download Length: 161 a.a. Molecular weight: 17662.45 Da Isoelectric Point: 8.9130
>T196303 WP_014386536.1 NZ_CP071394:82675-83157 [Klebsiella michiganensis]
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV
MGMRAPESLTAEHNIAEFCCQEPALNEWLKKKALRNHSTGISRVYVVCAENTNRVIGYYCLSSGSVHRNTVPGAYRRNAP
DVIPVIVLGRLAIDQAWAGNGLGAALLKDAIYRTQNIAFQVGVRALAVHALNEEVKCFYTRFGFEPSIVNTLTLLFPIKV
Download Length: 483 bp
>T196303 NZ_CP095086:c821625-821248 [Escherichia coli]
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATGCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA
ATGAACACATTACCCGATACACACGTACGGGAGGCATCGCATTGCCAGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGATCAGCACTACGGCCTTACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATGCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA
Antitoxin
Download Length: 93 a.a. Molecular weight: 10704.23 Da Isoelectric Point: 4.5271
>AT196303 WP_004902250.1 NZ_CP071394:82406-82684 [Klebsiella michiganensis]
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE
MKPDVREAPINIRAKTSQRDLIDMAASLVSKSRTDFMLEVACREAQDILLDQRLFLLNDNQFEAFIEELDAPITPERQAR
IDNLMNRKSPWE
Download Length: 279 bp
>AT196303 NZ_CP095086:c822083-821715 [Escherichia coli]
GTGTCAGACACACTCTCCGGGACAACGCATCCTGACGACAATCACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
GTGTCAGACACACTCTCCGGGACAACGCATCCTGACGACAATCACGACCGCCCCTGGTGGGGACTCCCCTGCACCGTGAC
GCCCTGTTTTGGGGCACGTCTGGTGCAGGAGGGTAACCGGTTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGGCAGT
TCAGCGACGCGGATGCGTACCATCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCAGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACGCTGTATGCAAAAAGGCTGACCTGCGAAGCCGACACCCTCGGCAGTTG
TGGCTACGTTTATATGGCTGTTTATCCGACGCCCGAAACGAAAAAGTAA
Similar Proteins
Only experimentally validated proteins are listed.
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
| Protein | Organism | Identities (%) | Coverage (%) | Ha-value |
|---|
Structures
Toxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A2A5MBI1 |
Antitoxin
| Source | ID | Structure |
|---|---|---|
| AlphaFold DB | A0A071LPN3 |