Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) /RES-TIGR02293
Location 5918..6828 Replicon plasmid pKmfe267-1
Accession NZ_CP071394
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name - Uniprot ID A0A5Q9LR54
Locus tag J1F62_RS27800 Protein ID WP_004181896.1
Coordinates 6358..6828 (+) Length 157 a.a.

Antitoxin (Protein)


Gene name - Uniprot ID A0A2J4RLY9
Locus tag J1F62_RS27795 Protein ID WP_004181895.1
Coordinates 5918..6361 (+) Length 148 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS27780 2551..4038 + 1488 WP_004178082.1 group II intron reverse transcriptase/maturase -
J1F62_RS27785 4124..4546 + 423 WP_004181893.1 translesion error-prone DNA polymerase V autoproteolytic subunit -
J1F62_RS27790 4546..5817 + 1272 WP_004181894.1 translesion error-prone DNA polymerase V subunit UmuC -
J1F62_RS27795 5918..6361 + 444 WP_004181895.1 DUF2384 domain-containing protein Antitoxin
J1F62_RS27800 6358..6828 + 471 WP_004181896.1 RES family NAD+ phosphorylase Toxin
J1F62_RS27805 6938..7198 - 261 WP_094966026.1 hypothetical protein -
J1F62_RS27810 7769..8950 - 1182 WP_004883380.1 IS481 family transposase -
J1F62_RS27815 9022..9180 + 159 WP_004181898.1 type I toxin-antitoxin system Hok family toxin -
J1F62_RS27820 9783..10757 + 975 WP_014386572.1 hypothetical protein -
J1F62_RS27825 10955..11332 - 378 WP_004181901.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid tet(A) / dfrA12 / aadA2 / qacE / sul1 / qnrB4 / blaDHA-1 / blaCTX-M-14 / sul2 / aph(3'')-Ib / aph(6)-Id / aac(3)-IId / catA2 / mph(A) - 1..251953 251953
- flank IS/Tn - - 7769..8950 1181


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

RES domain

(3-148)

Antitoxin

Antitoxin Xre-like, helix-turn-helix domain

(30-89)


Sequences

Toxin        


Download         Length: 157 a.a.        Molecular weight: 17549.81 Da        Isoelectric Point: 4.6155

>T196302 WP_004181896.1 NZ_CP071394:6358-6828 [Klebsiella michiganensis]
VIFYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPGNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIPFRFDSRLKPDRK

Download         Length: 471 bp

>T196302 NZ_CP095086:178884-178991 [Escherichia coli]
ATGACGCTCGCAGAGCTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAGCTGGCTGAACAAGCGGAAGTAA

Antitoxin


Download         Length: 148 a.a.        Molecular weight: 16536.87 Da        Isoelectric Point: 10.3013

>AT196302 WP_004181895.1 NZ_CP071394:5918-6361 [Klebsiella michiganensis]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVVNRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Download         Length: 444 bp

>AT196302 NZ_CP095086:c178827-178770 [Escherichia coli]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A5Q9LR54

Antitoxin

Source ID Structure
AlphaFold DB A0A2J4RLY9

References