Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) shpAB/BrnT-BrnA
Location 5185746..5186322 Replicon chromosome
Accession NZ_CP071393
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name shpA Uniprot ID A0A1D8JNH2
Locus tag J1F62_RS24350 Protein ID WP_042933975.1
Coordinates 5186035..5186322 (-) Length 96 a.a.

Antitoxin (Protein)


Gene name shpB Uniprot ID A0A0H3H591
Locus tag J1F62_RS24345 Protein ID WP_014227757.1
Coordinates 5185746..5186048 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS24330 5182586..5182921 + 336 WP_048262594.1 endoribonuclease SymE -
J1F62_RS24335 5183374..5184285 + 912 WP_207171899.1 acetamidase/formamidase family protein -
J1F62_RS24340 5184282..5185625 + 1344 WP_004128506.1 APC family permease -
J1F62_RS24345 5185746..5186048 - 303 WP_014227757.1 BrnA antitoxin family protein Antitoxin
J1F62_RS24350 5186035..5186322 - 288 WP_042933975.1 BrnT family toxin Toxin
J1F62_RS24355 5186574..5187017 - 444 WP_207171901.1 FosA family fosfomycin resistance glutathione transferase -
J1F62_RS24360 5187011..5187919 - 909 WP_207172836.1 LysR family transcriptional regulator -
J1F62_RS24365 5188007..5188789 + 783 WP_207171903.1 NAD(P)H-dependent oxidoreductase -
J1F62_RS24370 5188937..5189521 + 585 WP_004098242.1 TetR/AcrR family transcriptional regulator -
J1F62_RS24375 5189667..5190467 + 801 WP_046878031.1 winged helix-turn-helix domain-containing protein -
J1F62_RS24380 5190464..5190982 + 519 WP_004098240.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Ribonuclease toxin, BrnT, of type II toxin-antitoxin system

(7-88)

Antitoxin

BrnA antitoxin of type II toxin-antitoxin system

(38-93)


Sequences

Toxin        


Download         Length: 96 a.a.        Molecular weight: 11239.69 Da        Isoelectric Point: 8.5899

>T196301 WP_042933975.1 NZ_CP071393:c5186322-5186035 [Klebsiella michiganensis]
MPMEFEWDANKARSNLRKHGIRFEEAVLVFDDPRHLSRQDRYENGEYRWQTIGLVHGVIVIMVAHSVRFESGTEVIRIIS
ARKADSKERNRYEHG

Download         Length: 288 bp

>T196301 NZ_CP095084:66625-66774 [Escherichia coli]
ATGACGAAATATACCCTTATCGGGTTGCTCGCCGTGTGCGCCACGGTGTTGTGTTTTTCACTGATATTCAGGGAACGGTT
ATGTGAGCTGAATATTCACAGGGGAAATACAGTGGTGCAGGTAACTCTGGCCTACGAAGCACGGAAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11607.18 Da        Isoelectric Point: 9.4429

>AT196301 WP_014227757.1 NZ_CP071393:c5186048-5185746 [Klebsiella michiganensis]
MNMVKHKRDNPDTLTAKRDDELKALANKTDDEIDYSDIPATDDAQWSDAVRGKFYRPLKTQASVRIDADIMEWLKRPGKG
YQTRLNAILREAMIRDLNKK

Download         Length: 303 bp

>AT196301 NZ_CP095084:c66577-66521 [Escherichia coli]
GTACTTCATGCAGGCCTCACGAGTTAATGGATTAACAAGTGGGGTCTTCGCATTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1D8JNH2

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H591

References