Detailed information of TA system TA196299

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): yeeUV (cbtA-cbeA)/CbtA-CbeA
• Location: 4701187..4701962
• Replicon: chromosome
• Accession: NZ_CP071393
• Organism: Klebsiella michiganensis strain Kmfe267

Toxin (Protein)

• Gene name: yeeV
• Uniprot ID: -
• Locus tag: J1F62_RS22140
• Protein ID: WP_131633358.1
• Coordinates: 4701585..4701962 (+)
• Length: 126 a.a.

Antitoxin (Protein)

• Gene name: yeeU
• Uniprot ID: -
• Locus tag: J1F62_RS22135
• Protein ID: WP_207172832.1
• Coordinates: 4701187..4701534 (+)
• Length: 116 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F62_RS22100	4697121..4697756	+	636	WP_131633367.1	hypothetical protein	-
J1F62_RS22105	4697792..4698244	+	453	WP_131633366.1	hypothetical protein	-
J1F62_RS22110	4698241..4698720	+	480	WP_131633365.1	hypothetical protein	-
J1F62_RS22115	4698840..4699658	+	819	WP_131633364.1	DUF945 domain-containing protein	-
J1F62_RS22120	4699928..4700398	+	471	WP_131633362.1	antirestriction protein	-
J1F62_RS22125	4700410..4700889	+	480	WP_131633361.1	DNA repair protein RadC	-
J1F62_RS22130	4700910..4701131	+	222	WP_131633360.1	DUF987 domain-containing protein	-
J1F62_RS22135	4701187..4701534	+	348	WP_207172832.1	type IV toxin-antitoxin system YeeU family antitoxin	Antitoxin
J1F62_RS22140	4701585..4701962	+	378	WP_131633358.1	TA system toxin CbtA family protein	Toxin
J1F62_RS22145	4701959..4702369	+	411	WP_131633357.1	malate transporter	-
J1F62_RS22150	4702339..4703319	+	981	WP_131633356.1	EAL domain-containing protein	-
J1F62_RS22155	4703584..4704057	-	474	WP_165500690.1	transposase	-
J1F62_RS22160	4704490..4705104	-	615	WP_131633355.1	inovirus Gp2 family protein	-
J1F62_RS22165	4705408..4705602	-	195	WP_207172833.1	helix-turn-helix domain-containing protein	-
J1F62_RS22170	4706102..4706863	-	762	WP_131633353.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Genomic island	-	-	4687475..4712243	24768

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• CbtA toxin of type IV toxin-antitoxin system (1-112)

Antitoxin

• Antitoxin CbeA (4-110)

Sequences

Toxin

Protein

• Length: 126 a.a.
• Molecular weight: 14295.17 Da
• Isoelectric Point: 6.6357

>T196299 WP_131633358.1 NZ_CP071393:4701585-4701962 [Klebsiella michiganensis]
MQTQPLSSTQEATAHPSPVEIWQRLLSHLLDRHYGLTLNDKPFGNDCVIQEHIDAGISLCDAVNFIVEKYDLVRIDRHGF
STETQLPRLTSIDILRARKTTGLMTRNDYRTVTDITTGKYREVQP

Nucleotide

• Length: 378 bp

>T196299 NZ_CP095083:4537967-4538224 [Escherichia coli]
ATGAATTTATCCCGTCAGGAACAACATACCTTACACGTTCTCGCTAAAGGTAGACGTATTGCGCACGTCCGCGATTCTTC
AGGCCGCGTCACTTCCGTTGAATGCTACAGCCGCGAAGGGCTGTTGCTGACCGACTGCACGCTCGCCGTCTTCAAAAAAC
TCAAAACCAAAAAACTTATCAAGTCCGTCAATGGCCAGCCCTATCGAATCAACACCACCGAGCTGAATAAAGTTCGCGCT
CAGCTCGATAATCGCTAA

Antitoxin

Protein

• Length: 116 a.a.
• Molecular weight: 12779.76 Da
• Isoelectric Point: 7.3501

>AT196299 WP_207172832.1 NZ_CP071393:4701187-4701534 [Klebsiella michiganensis]
IAEPWWGLKRSITPCFGARLVQAGNRLHYLADRASITGQFSDADLRHLDQAFPLLLKQLELMLKQLELMLTSGELNPRHQ
HCVTLYAKGLTCEADSLGSHGYVYLAIYPTPAATA

Nucleotide

• Length: 348 bp

>AT196299 NZ_CP095083:4538236-4538781 [Escherichia coli]
ATGACAGTACACCACTTCACTTTTCATATCACAGATAAAAGTGATGCCAGTGATATCCGGGAAGTCGAAACCCGCGCATT
TGGCTTCAGCAAAGAAGCCGATTTAGTGGCCTCTTTACTTGAGGATGAAAGCGCGCGCCCCGCGTTATCGCTGCTAGCCC
GGTATGAAGGCAAAGCCGTCGGGCATATTTTATTTACCCGGGCAACCTTCAAAGGAGAAATGGATTCACCGTTGATGCAC
ATCCTCGCTCCCCTGGCGGTCATACCGGAATACCAAGGGATGGGCGTGGGAGGACGGTTGATACGCACTGGCATCGAGCA
CCTGAGGTTGATGGGCTGCCAGACGGTTTTTGTTCTCGGCCACGCCACCTATTACCCCCGACATGGGTTCGAACCCTGTG
CCGGAGACAAAGGCTATCCGGCACCTTATCCCATCCCGGAGGAGCACAAAGCCTGCTGGATGATGCAATCACTCACCGCT
CAGCCCATGACCCTGACCGGCCACATCCGGTGCGCAGACCCTGATGAAACCGGAGCACTGACGTGA