Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) Hha-TomB/-
Location 4488539..4489158 Replicon chromosome
Accession NZ_CP071393
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name Hha Uniprot ID H3N9D8
Locus tag J1F62_RS21100 Protein ID WP_004099646.1
Coordinates 4488940..4489158 (+) Length 73 a.a.

Antitoxin (Protein)


Gene name TomB Uniprot ID A0A0J2I7Z1
Locus tag J1F62_RS21095 Protein ID WP_025107145.1
Coordinates 4488539..4488913 (+) Length 125 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS21085 4483695..4484888 + 1194 WP_004136054.1 multidrug efflux RND transporter periplasmic adaptor subunit AcrA -
J1F62_RS21090 4484911..4488057 + 3147 WP_014228207.1 multidrug efflux RND transporter permease subunit -
J1F62_RS21095 4488539..4488913 + 375 WP_025107145.1 Hha toxicity modulator TomB Antitoxin
J1F62_RS21100 4488940..4489158 + 219 WP_004099646.1 hemolysin expression modulator Hha Toxin
J1F62_RS21105 4489321..4489887 + 567 WP_032748289.1 maltose O-acetyltransferase -
J1F62_RS21110 4490014..4490484 + 471 WP_014228205.1 YlaC family protein -
J1F62_RS21115 4490459..4491913 - 1455 WP_134899451.1 PLP-dependent aminotransferase family protein -
J1F62_RS21120 4492015..4492713 + 699 WP_134899183.1 GNAT family N-acetyltransferase -
J1F62_RS21125 4492710..4492850 - 141 WP_003859006.1 type B 50S ribosomal protein L36 -
J1F62_RS21130 4492850..4493113 - 264 WP_004848323.1 type B 50S ribosomal protein L31 -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Haemolysin expression modulating, HHA

(13-69)

Antitoxin

Biofilm formation regulator YbaJ

(1-115)


Sequences

Toxin        


Download         Length: 73 a.a.        Molecular weight: 8640.05 Da        Isoelectric Point: 8.9008

>T196298 WP_004099646.1 NZ_CP071393:4488940-4489158 [Klebsiella michiganensis]
MSDKTLTKIDYLMRLRRCQTIDTLERVIEKNKYELSDNELAVFYSAADHRLAELTMNKLYDKIPPSVWKFIR

Download         Length: 219 bp

>T196298 NZ_CP095083:4484575-4484916 [Escherichia coli]
ATGACTGACACGCATTCTATTGCACAACCGTTCGAAGCAGAAGTCTCCCCGGCAAATAACCGTCATGTCACCGTCGGTTA
TGCGAGTCGCTACCCGGATTACAGCCGTATTCCCGCCATCACCCTGAAAGGTCAGTGGCTGGAAGCCGCCGGTTTTGCCA
CTGGCACGGCGGTAGATGTCAAAGTGATGGAAGGCTGTATTGTCCTCACCGCCCAACCACCCGCCGCCGAGGAGAGCGAA
CTGATGCAGTCGCTACGCCAGGTGTGCAAACTGTCGGCGCGTAAACAAAAGCAGGTGCAGGCGTTTATTGGAGTGATTGC
CGGTAAACAGAAAGTCGCGTAA

Antitoxin


Download         Length: 125 a.a.        Molecular weight: 14366.15 Da        Isoelectric Point: 4.8989

>AT196298 WP_025107145.1 NZ_CP071393:4488539-4488913 [Klebsiella michiganensis]
MDEYSPKRHDIAQLKFLCETLYHDCLANLEESNHGWVNDPTSAVNLQLNELIEHIAAFALNYKIKYAEDNKLITQIDEYL
DDTFVLFSNYGINSADLQKWRKSGNRLFRCFVNACRENPASLSC

Download         Length: 375 bp

>AT196298 NZ_CP095083:c4484580-4484504 [Escherichia coli]
AGTCATAACTGCTATTCTCCAGGAATAGTGATTGTGATTAGCGATGCGGGTGTGTTGGCGCACATCCGCACCGCGCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H713

Antitoxin

Source ID Structure
AlphaFold DB A0A0J2I7Z1

References