Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type IV Classification (family/domain) cptAB/Cpta(toxin)
Location 861172..861829 Replicon chromosome
Accession NZ_CP071393
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name cptA Uniprot ID J5U333
Locus tag J1F62_RS04150 Protein ID WP_004854060.1
Coordinates 861419..861829 (+) Length 137 a.a.

Antitoxin (Protein)


Gene name cptB Uniprot ID H3N295
Locus tag J1F62_RS04145 Protein ID WP_004124953.1
Coordinates 861172..861438 (+) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS04130 856406..856831 - 426 WP_004854067.1 PTS sugar transporter subunit IIA -
J1F62_RS04135 856952..859750 - 2799 WP_014226794.1 transcriptional regulator DagR -
J1F62_RS04140 859944..860927 - 984 WP_014226793.1 tRNA-modifying protein YgfZ -
J1F62_RS04145 861172..861438 + 267 WP_004124953.1 FAD assembly factor SdhE Antitoxin
J1F62_RS04150 861419..861829 + 411 WP_004854060.1 protein YgfX Toxin
J1F62_RS04155 861838..862359 - 522 WP_014226792.1 flavodoxin FldB -
J1F62_RS04160 862481..863377 + 897 WP_004105555.1 site-specific tyrosine recombinase XerD -
J1F62_RS04165 863400..864113 + 714 WP_004854054.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -
J1F62_RS04170 864119..865852 + 1734 WP_032751182.1 single-stranded-DNA-specific exonuclease RecJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Inner membrane protein YgfX

(3-131)

Antitoxin

Flavinator of succinate dehydrogenase

(7-79)


Sequences

Toxin        


Download         Length: 137 a.a.        Molecular weight: 16106.97 Da        Isoelectric Point: 10.9455

>T196288 WP_004854060.1 NZ_CP071393:861419-861829 [Klebsiella michiganensis]
VVLWQSDLRISWRAQWFSLLMHGVVAALVLLMPWPLSYTPLWLILLSLVVFDCVRSQRRIHARQGEIKLLIDSRLRWQKA
EWDIIGTPWVINSGMLLRLRNTENQRTQHLWVAADSMDAGEWRDLRRLVLLKPTQE

Download         Length: 411 bp

>T196288 NZ_CP095083:2199531-2199634 [Escherichia coli]
GGCAAGGCAACTAAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATAC
AGGAATCGTGTTCGGTCTCTTTTT

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 10411.88 Da        Isoelectric Point: 5.1296

>AT196288 WP_004124953.1 NZ_CP071393:861172-861438 [Klebsiella michiganensis]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDTLSDGDKALFIRLLENDDPDLFNWLMNHGEPADAELQRMVKLIQTRN
RERGPVAI

Download         Length: 267 bp

>AT196288 NZ_CP095083:c2199672-2199401 [Escherichia coli]
AAAGTCAGCGAAGGGAATGCTTCTGGCTTTTAACAGATAAAAAGAGACCGAACACGATTCCTGTATTCGGTCCAGGGAAA
TGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTGGCATTAATGCAGGCTTAGTTGCCTTGCCCTTTAAGAATAGATGACG
ACGCCAGGTTTTCCAGTTTGCGTGCAAAATGGTCAATAAAAAGCGCGGTGGTCATCAGCTTAAATGTTAAAAACCGCCCG
TTCTGGTGAAAGAACTGAGGCGGTTTTTTTAT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GYA5

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H3L9

References