Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) ccdAB/CcdA(antitoxin)
Location 67007..67554 Replicon chromosome
Accession NZ_CP071393
Organism Klebsiella michiganensis strain Kmfe267

Toxin (Protein)


Gene name ccdB Uniprot ID A0A0H3H5T3
Locus tag J1F62_RS00330 Protein ID WP_014227299.1
Coordinates 67007..67315 (-) Length 103 a.a.

Antitoxin (Protein)


Gene name ccdA Uniprot ID A0A0H3H0P7
Locus tag J1F62_RS00335 Protein ID WP_014227298.1
Coordinates 67318..67554 (-) Length 79 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F62_RS00305 62923..63234 - 312 WP_014227304.1 PTS sugar transporter subunit IIB -
J1F62_RS00310 63529..64470 + 942 WP_014227303.1 LacI family transcriptional regulator -
J1F62_RS00315 64494..64787 - 294 WP_014227302.1 hypothetical protein -
J1F62_RS00320 64997..65950 + 954 WP_207172081.1 Rpn family recombination-promoting nuclease/putative transposase -
J1F62_RS00325 65954..66856 - 903 WP_009654083.1 DMT family transporter -
J1F62_RS00330 67007..67315 - 309 WP_014227299.1 CcdB family protein Toxin
J1F62_RS00335 67318..67554 - 237 WP_014227298.1 type II toxin-antitoxin system CcdA family antitoxin Antitoxin
J1F62_RS00340 67660..69093 - 1434 WP_032718833.1 PTS N-acetylmuramic acid transporter subunit IIBC -
J1F62_RS00345 69118..70020 - 903 WP_032752002.1 N-acetylmuramic acid 6-phosphate etherase -
J1F62_RS00350 70181..71347 - 1167 WP_064406020.1 multidrug effflux MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin CcdB

(2-101)

Antitoxin

Post-segregation antitoxin CcdA

(8-78)


Sequences

Toxin        


Download         Length: 103 a.a.        Molecular weight: 11725.57 Da        Isoelectric Point: 7.2729

>T196286 WP_014227299.1 NZ_CP071393:c67315-67007 [Klebsiella michiganensis]
MQYYVYKNTGRIAAYPYLLDVQSDIIGKRNTRVVIPLFPLKNYKGPRADRLTPLVTVEGEEYVVMTHELASIPHRVLGEE
VCNLNHQREVVKASMDFLFDGI

Download         Length: 309 bp

>T196286 NZ_CP095083:c804451-804074 [Escherichia coli]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCATCACCATCAGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCGTCGCAGTTAATCAACAGCATTGATATTCTCCGGGCACGCCGGGCAACCGGCCTGATGACCCGCGA
TAACTACAGAACGGTAAATAACATTACCCGGGGGAAGCATCCGGAAGCGAAACAATGA

Antitoxin


Download         Length: 79 a.a.        Molecular weight: 8783.92 Da        Isoelectric Point: 5.7928

>AT196286 WP_014227298.1 NZ_CP071393:c67554-67318 [Klebsiella michiganensis]
MPAAKRNKQNVSVTLDPGLVKQAREVGINLSLTLTNALKSEIHSLEIARWQKENAQAIDYLNNLTEENGLLSDEYRTF

Download         Length: 237 bp

>AT196286 NZ_CP095083:c804875-804498 [Escherichia coli]
GTGTCAGACACACTCCCCGGGACAACGCCTCCCGACGACAATCACGACCGCCCCTGGTGGGGGCTGCCCTGCACCGTGAC
GTCCTGTTTCGGGGCCCGACTGGTACAGGAGGGTAACCGCCTGCATTACCTTGCAGACCGCGCCGGTATCAGAGGCAGGT
TCAGCGACGTAGATGCGTACCACCTGGACCAGGCCTTTCCGCTGCTGATGAAACAACTGGAACTCATGCTCACCGGCGGT
GAACTGAATCCCCGCCATCAGCATACCGTCACACTGTATGCGAAAGGGCTGACCTGCGAAGCCGACACCCTCGGCTCCTG
TGGTTACGTTTATCTGGCTGTTTATCCGACACCGGCAGCGCCCGCAACCACCGTATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3H5T3

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3H0P7

References