Detailed information of TA system TA196283

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): /RES-TIGR02293
• Location: 29232..30142
• Replicon: plasmid unnamed1
• Accession: NZ_CP071386
• Organism: Klebsiella pneumoniae strain wxc2020

Toxin (Protein)

• Gene name: -
• Uniprot ID: -
• Locus tag: J1F26_RS25950
• Protein ID: WP_032413375.1
• Coordinates: 29672..30142 (+)
• Length: 157 a.a.

Antitoxin (Protein)

• Gene name: -
• Uniprot ID: A0A7H4PRG4
• Locus tag: J1F26_RS25945
• Protein ID: WP_032413443.1
• Coordinates: 29232..29675 (+)
• Length: 148 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F26_RS25915	24916..25587	-	672	WP_159226239.1	alpha/beta hydrolase	-
J1F26_RS25920	25678..26646	-	969	WP_077268726.1	IS5-like element IS903B family transposase	-
J1F26_RS25925	27062..27610	+	549	WP_023320124.1	GNAT family N-acetyltransferase	-
J1F26_RS25930	27733..28314	-	582	WP_077254766.1	transposase	-
J1F26_RS25935	28334..28519	+	186	WP_071571073.1	DUF2188 domain-containing protein	-
J1F26_RS25940	28620..29132	+	513	Protein_30	DUF4113 domain-containing protein	-
J1F26_RS25945	29232..29675	+	444	WP_032413443.1	DUF2384 domain-containing protein	Antitoxin
J1F26_RS25950	29672..30142	+	471	WP_032413375.1	RES family NAD+ phosphorylase	Toxin
J1F26_RS25955	30252..30512	-	261	WP_017901409.1	hypothetical protein	-
J1F26_RS25960	31084..31461	-	378	Protein_34	integrase core domain-containing protein	-
J1F26_RS25965	31535..31728	+	194	Protein_35	transposase	-
J1F26_RS25970	31851..32801	+	951	WP_207604423.1	IS5 family transposase	-
J1F26_RS25975	32854..33279	+	426	Protein_37	hypothetical protein	-
J1F26_RS25980	33280..33435	-	156	Protein_38	transposase	-
J1F26_RS25985	34171..34263	-	93	Protein_39	hypothetical protein	-
J1F26_RS25990	34305..34841	+	537	WP_032437437.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	dfrA12 / aadA2 / qacE / sul1 / armA / msr(E) / mph(E) / mph(A) / blaDHA-1 / qnrB4 / aph(3'')-Ib / aph(6)-Id / sul2	-	1..195272	195272
	-	inside	IScluster/Tn	-	-	21487..37320	15833

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• RES domain (3-148)

Antitoxin

• Antitoxin Xre (16-147)

Sequences

Toxin

Protein

• Length: 157 a.a.
• Molecular weight: 17547.79 Da
• Isoelectric Point: 4.5152

>T196283 WP_032413375.1 NZ_CP071386:29672-30142 [Klebsiella pneumoniae]
VILYRLTKTKYLSTAWTGYGAKEAGGRWNSVGVSMVYVSETASLTMLETLVHLHAAQIMDSFTLLSIDVPDELIQSANMD
ELPDNWADEDAPQELADYGDAWSFTRSSVALRVPSALSPVEFNYLLNPEHPEFYGIVQKAQQIAFRFDSRLKPDRK

Nucleotide

• Length: 471 bp

>T196283 NZ_CP095083:c641764-641300 [Escherichia coli]
ATGGATTTTCCACAAAGGGTTAATGGTTGGGCGCTATATGCTCATCCCTGTTTTCAGGAAACCTACGACGCTTTAGTTGC
CGAAGTCGAGACATTAAAGGGAAAAGATCCTGAAAATTATCAGAGAAAAGCCGCCACAAAGTTATTGGCGGTAGTCCATA
AAGTGATTGAGGAGCATATCACGGTCAATCCATCATCACCGGCATTCCGTCATGGCAAGTCGTTAGGCTCTGGGAAAAAT
AAAGACTGGTCACGGGTAAAATTTGGTGCTGGTCGTTATCGTCTCTTCTTTCGTTATAGTGAAAAAGAGAAAGTCATCAT
TCTGGGATGGATGAACGATGAAAACACTCTGCGCACCTACGGTAAAAAAACAGATGCCTATACCGTATTCAGCAAAATGT
TAAAAAGAGGACATCCTCCTGCCGACTGGGAAACCCTCACCCGAGAAACAGAAGAAACCCATTGA

Antitoxin

Protein

• Length: 148 a.a.
• Molecular weight: 16508.82 Da
• Isoelectric Point: 10.3013

>AT196283 WP_032413443.1 NZ_CP071386:29232-29675 [Klebsiella pneumoniae]
MKTFSLSSTPARPQRLWQVAGLNNADGVALLGQINEGLDGKVANRITDWARITQNDLRKMSGIPSTTFSRSVKARFNPEQ
SERLVRIIRVIDRAVDLFEGDREAAQKWLNEPNRALSWKVPADLMASETGAYEVIKLITRLEHGVYS

Nucleotide

• Length: 444 bp

>AT196283 NZ_CP095083:c642099-641764 [Escherichia coli]
ATGCCCGCTAATGCTCGCTCTCACGCTGTACTGACCACTGAATCAAAGGTCACGATACGCGGACAAACAACTATCCCCGC
GCCAGTGCGTGAGGCCTTAAAACTGAAGCCAGGCCAGGACAGCATTCATTACGAAATTCTGCCTGGTGGGCAAGTATTTA
TGTGCCGACTGGGAGATGAACAGGAGGATCATACTATGAATGCATTTTTGCGTTTTCTGGATGCAGATATCCAGAACAAC
CCGCAAAAAACTCGTCCATTCAACATTCAACAAGGAAAGAAACTTGTCGCTGGCATGGACGTCAACATTGATGATGAGAT
TGGCGACGACGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A7H4PRG4