Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 5207479..5208104 Replicon chromosome
Accession NZ_CP071385
Organism Klebsiella pneumoniae strain wxc2020

Toxin (Protein)


Gene name vapC Uniprot ID A0A483GDF6
Locus tag J1F26_RS25425 Protein ID WP_004187928.1
Coordinates 5207479..5207862 (-) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J1F26_RS25430 Protein ID WP_004150355.1
Coordinates 5207862..5208104 (-) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F26_RS25410 5204845..5205747 + 903 WP_002882822.1 formate dehydrogenase subunit beta -
J1F26_RS25415 5205744..5206379 + 636 WP_002882818.1 formate dehydrogenase cytochrome b556 subunit -
J1F26_RS25420 5206376..5207305 + 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J1F26_RS25425 5207479..5207862 - 384 WP_004187928.1 type II toxin-antitoxin system VapC family toxin Toxin
J1F26_RS25430 5207862..5208104 - 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
J1F26_RS25435 5208298..5209215 + 918 WP_004187929.1 alpha/beta hydrolase -
J1F26_RS25440 5209229..5210170 - 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J1F26_RS25445 5210215..5210652 - 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
J1F26_RS25450 5210649..5211509 - 861 WP_004146232.1 virulence factor BrkB family protein -
J1F26_RS25455 5211503..5212102 - 600 WP_004151865.1 glucose-1-phosphatase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-110)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14407.64 Da        Isoelectric Point: 7.3178

>T196282 WP_004187928.1 NZ_CP071385:c5207862-5207479 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFTGIPGVVTPYEIHPE

Download         Length: 384 bp

>T196282 NZ_CP095083:150199-150384 [Escherichia coli]
GTGAAAAGTGCAGACGTGATCGCCATCCTGAAGCAGCATGGCTGGGAACATATTCGAACCCGTGGCAGTCATCATCAGTT
CCGCCACCCTGTTCATCCGGGTCTGGTGACGGTTCCGCACCCTAAAAAAGATATTAAGCCCGGTACGCTGGCGCAAATTG
GGCGTCAGGCTGGTTTAACATTTTAA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT196282 WP_004150355.1 NZ_CP071385:c5208104-5207862 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT196282 NZ_CP095083:150401-150811 [Escherichia coli]
ATGTTTTATCCCGCCTATATCCACAGTGATCCTGACGGCAGCGCCAGCGGTTTTTTCCCTGATGTTCCAGGCTGTTATTT
TGCTGGTGATACGCTGGACAACGCTTTCCAGGATGCGCGAAGTGCACTGGTCGCGCACTTCGAAACCTTATGCGAGATGA
ATGAAGAGTTGCCGCTTCCAGGCTCTGTGGAAACACATCTGGTCCAGCGTGCGCAGGACTTTATTGGCGGGCAATGGTTG
CTGGTGGATATCAATATGAATCAGTTCGAAGGTCGGGCTGAACGTATCAATATTACGATGCCGAAAAGGTTACTGAACAA
GATTGATACCTACGTGCGCAATAACCCAGACTACGCCAACAGAAGTGCGTTTCTGGCAGAAGCTGCCCGGCGTGTATTGC
CGGGCGTTTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483GDF6

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References