Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) kacAT/DUF1778(antitoxin)
Location 4558057..4558867 Replicon chromosome
Accession NZ_CP071385
Organism Klebsiella pneumoniae strain wxc2020

Toxin (Protein)


Gene name KacT Uniprot ID A0A483G813
Locus tag J1F26_RS22285 Protein ID WP_023279404.1
Coordinates 4558057..4558590 (-) Length 178 a.a.

Antitoxin (Protein)


Gene name KacA Uniprot ID J2E9Q7
Locus tag J1F26_RS22290 Protein ID WP_002887278.1
Coordinates 4558601..4558867 (-) Length 89 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J1F26_RS22280 4556888..4558009 + 1122 WP_002887282.1 cupin domain-containing protein -
J1F26_RS22285 4558057..4558590 - 534 WP_023279404.1 GNAT family N-acetyltransferase Toxin
J1F26_RS22290 4558601..4558867 - 267 WP_002887278.1 DUF1778 domain-containing protein Antitoxin
J1F26_RS22295 4558970..4560403 - 1434 WP_004192214.1 Cu(+)/Ag(+) sensor histidine kinase -
J1F26_RS22300 4560393..4561076 - 684 WP_002887273.1 copper response regulator transcription factor CusR -
J1F26_RS22305 4561248..4562633 + 1386 WP_004192218.1 efflux transporter outer membrane subunit -
J1F26_RS22310 4562651..4562995 + 345 WP_004192220.1 cation efflux system protein CusF -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

GNAT domain

(91-151)

Antitoxin

Vibrio phage ICP1, Orf50

(2-86)


Sequences

Toxin        


Download         Length: 178 a.a.        Molecular weight: 19809.71 Da        Isoelectric Point: 6.2369

>T196279 WP_023279404.1 NZ_CP071385:c4558590-4558057 [Klebsiella pneumoniae]
MEQQLTIEMIADAFSYDITGFDCGEEALNTFLKEHLKRQHDGQILRGYALVSGDTVPRLLGYYTLSGSCFERGMLPSKTQ
QKKIPYQNAPSVTLGRLAIDKSVQGQGWGEMLVAHAMRVVWGASKAVGIYGLFVEALNEKAKAFYLRLGFIQLVDKNSNL
LFYPTKSIEQLFTDDES

Download         Length: 534 bp

>T196279 NZ_CP095074:2940474-2940824 [Halobacillus sp. SSTM10-2]
GTGATAGTCAAACGAGGAGACGTTTATTTCGCTGATTTGTCCCCAGTTGTAGGATCAGAACAAGGAGGAGTGCGTCCTGT
TCTGATTTTACAAAATGATATCGGGAATCGTTTCAGTCCCACAGTTATTATTGCAGCGATTACTGCGCAAATACAAAAGG
CAAAACTTCCTACACATGTTGAGATTAATGCTGAAAAATATGGTTTCGAACGCAATTCTGTTATTCTGCTGGAACAAATC
AGAACGATTGATAAACAACGTTTGACCGATAAGATCACACAACTAGATGGATCTATGATGCAACGTGTAAATGAATCGTT
GCAAATCAGCCTTGGTCTAATCGACTTTTAA

Antitoxin


Download         Length: 89 a.a.        Molecular weight: 9996.46 Da        Isoelectric Point: 10.1757

>AT196279 WP_002887278.1 NZ_CP071385:c4558867-4558601 [Klebsiella pneumoniae]
MPALKKQRIDLRLTDDDKSIIEEAAAISNQTITQFVVASASERAAEVIEQHRRMVLNEQSWSLVMEAITQPPAPNDRLKR
AAKRLQTR

Download         Length: 267 bp

>AT196279 NZ_CP095074:2940187-2940468 [Halobacillus sp. SSTM10-2]
ATGGTTTTGTCCGACAACATGCAGGAGATTGTCATTCGTATTCCAGATAGTCTGCTAAACGAAGTGGATGGTCATGTTCA
ACTCGAAAACAGTGATCGGAGCGATTTCATGTGTAAAGCAACGAAAATGTACTTGCGGGAAGAGAAACAACGCTATATTA
GAGAATCCATGCGTCGTGGATATATGGAAATGGCGAGAATTAATCTAACTATCGCTTCAGAAGCTTTTCAGGCCGAAGAG
GAGGCAGATCACACCCTGGAGCAATTAGTGAGCGGGGTGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A483G813

Antitoxin

Source ID Structure
PDB 5ZGN
AlphaFold DB A0A0H3GLZ1

References