Detailed information of TA system TA196277

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 2639879..2641467
• Replicon: chromosome
• Accession: NZ_CP071385
• Organism: Klebsiella pneumoniae strain wxc2020

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: A0A486KW17
• Locus tag: J1F26_RS12970
• Protein ID: WP_004190166.1
• Coordinates: 2639879..2641201 (-)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: A0A0H3GXD8
• Locus tag: J1F26_RS12975
• Protein ID: WP_002904790.1
• Coordinates: 2641201..2641467 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J1F26_RS12955	2634935..2637436	+	2502	WP_004190161.1	fimbrial biogenesis outer membrane usher protein	-
J1F26_RS12960	2637424..2638428	+	1005	WP_004190163.1	type 1 fimbrial protein	-
J1F26_RS12965	2638570..2639862	+	1293	WP_002904794.1	glycoside hydrolase family 10 protein	-
J1F26_RS12970	2639879..2641201	-	1323	WP_004190166.1	type II toxin-antitoxin system HipA family toxin	Toxin
J1F26_RS12975	2641201..2641467	-	267	WP_002904790.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J1F26_RS12980	2641732..2642058	-	327	WP_004143476.1	hydroxyisourate hydrolase	-
J1F26_RS12985	2642055..2642555	-	501	WP_004190169.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J1F26_RS12990	2642533..2643912	-	1380	WP_004184021.1	purine permease	-
J1F26_RS12995	2644258..2645412	+	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J1F26_RS13000	2645393..2646316	-	924	WP_004190172.1	LysR family hpxDE operon transcriptional regulator HpxR	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA, N-terminal subdomain 1 (4-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T196277 WP_004190166.1 NZ_CP071385:c2641201-2639879 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTTLLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIIATPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T196277 NZ_CP095072:c621742-621392 [Gracilibacillus sp. SSWR10-1]
TTGATTGTGAAAAGAGGCGACGTATATTTCGCCGATCTTTCCCCTGTTGTGGGATCTGAACAGGGTGGAGTACGTCCCGT
ATTAGTATTACAAAATGATATTGGTAACCGTTTTAGTCCAACTGTTATTGTAGCAGCAATCACAGCTCAAATTCAAAAGG
CGAAATTACCGACACATGTCGAAATAGATGCAGAAAAGTACGGTTTTGAGCGTAATTCGGTTATTTTATTAGAACAAATC
CGTACCATTGATAAACAACGATTAACTGATAAAATAACTCAATTAGACAGTAACATGATGGAACGTATTGATAGAGGATT
AGAAATTAGTTTAGGGTTAACTGATTTCTAA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9906.27 Da
• Isoelectric Point: 8.5912

>AT196277 WP_002904790.1 NZ_CP071385:c2641467-2641201 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMTLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT196277 NZ_CP095072:c622027-621746 [Gracilibacillus sp. SSWR10-1]
ATGGTTTTGTCTGAAGACTTGCAAGAAGTTGCTGTGAGATTACCTAAAAACCTGCTGAATGAGGTGGACGGCTTGATGAA
AAGCGAAGATAGCAATTTAAGTGATTTTATTTGCCAAGCAACCAGAACGTATTTGCGTGAAACAAAAAAACGTCATATTC
AAGAATCCATGCAAAGAGGATATATGGAAATGGCGAAAATTAATTTGAATATTGCTTCGGAAGCTTTTCAGGCGGAAGAG
GAGGCAGAAAACACCCTTGAGCGCTTAGTGAGCGGGGTGTAG

Structures

Toxin

• AlphaFold DB: A0A486KW17

Antitoxin

• AlphaFold DB: A0A0H3GXD8