Detailed information of TA system TA196264

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 4020770..4021356
• Replicon: chromosome
• Accession: NZ_CP071383
• Organism: Leclercia sp. 4-9-1-25

Toxin (Protein)

• Gene name: doc
• Uniprot ID: A0A0M3F019
• Locus tag: JZ655_RS19555
• Protein ID: WP_040078242.1
• Coordinates: 4020770..4021138 (-)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: -
• Locus tag: JZ655_RS19560
• Protein ID: WP_207292542.1
• Coordinates: 4021135..4021356 (-)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
JZ655_RS19535	4016064..4017134	-	1071	WP_040078246.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-
JZ655_RS19540	4017136..4017981	-	846	WP_207292541.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
JZ655_RS19545	4017978..4018865	-	888	WP_040078244.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
JZ655_RS19550	4018982..4020298	-	1317	WP_040078243.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
JZ655_RS19555	4020770..4021138	-	369	WP_040078242.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
JZ655_RS19560	4021135..4021356	-	222	WP_207292542.1	type II toxin-antitoxin system prevent-host-death family antitoxin	Antitoxin
JZ655_RS19565	4021443..4022156	-	714	WP_046886786.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
JZ655_RS19570	4022158..4022925	-	768	WP_046886787.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
JZ655_RS19575	4022922..4024199	-	1278	WP_207292543.1	high-affinity branched-chain amino acid ABC transporter permease LivM	-
JZ655_RS19580	4024196..4025122	-	927	WP_040078237.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
JZ655_RS19585	4025169..4026278	-	1110	WP_040078235.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	4015324..4032941	17617

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (6-112)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-52)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13650.95 Da
• Isoelectric Point: 6.7177

>T196264 WP_040078242.1 NZ_CP071383:c4021138-4020770 [Leclercia sp. 4-9-1-25]
MTLQFISAEEIVLFHDKLLSVTPGVAGMPDPGRAEALLYRVLNKYEYEGVTDVWMLAAMHLLAISRGHIFNDGNKRTALF
ITLLFLKRNGISLKANPDYVDMTVEAAAGRLTLDEVVQRLRC

Nucleotide

• Length: 369 bp

>T196264 NZ_CP095023:c3705173-3704745 [Mycobacterium tuberculosis]
ATGCGAGCGCTGTTGGACGTCAACGTGCTACTGGCGCTGTTGGACCGCGATCACGTCGACCACGAGCGCGCCCGAGCGTG
GATCACCGGGCAGATCGAGCGGGGATGGGCTTCCTGTGCGATCACCCAAAACGGGTTCGTCCGCGTCATCAGCCAACCGC
GCTATCCCAGCCCAATCTCGGTAGCCCATGCCATCGACTTGTTGGCTCGTGCGACGCACACGCGCTATCACGAGTTCTGG
TCCTGCACCGTCAGCATTCTCGACTCAAAGGTCATCGATCGTTCCCGCCTGCACAGCCCCAAACAGGTCACCGACGCGTA
CTTGCTGGCCCTCGCGGTCGCTCATGATGGCCGATTTGTCACCTTCGATCAGTCGATTGCGTTGACTGCGGTTCCTGGCG
CAACCAAACAGCACCTGGCAACGCTGTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8041.04 Da
• Isoelectric Point: 5.6663

>AT196264 WP_207292542.1 NZ_CP071383:c4021356-4021135 [Leclercia sp. 4-9-1-25]
MRTMNYSEARQNLASALESAAAGTPVTISRRGHKSAVIISVEEFERYQTAKLDAEFDAIMGIHGSEIRELADK

Nucleotide

• Length: 222 bp

>AT196264 NZ_CP095023:c3705419-3705177 [Mycobacterium tuberculosis]
ATGCGCACCACGTTGTCCATCGACGACGATGTATTGCTGGCCGTGAAGGAACGGGCACGCAGGGAGAAGCGCACCGCGGG
TGAAATCCTGTCGGATCTGGCCCGGCAAGCGTTAACCAACCAGAATCCCCAGCCTGCGGCGAGCCAGGAGGACGCCTTTC
ATGGCTTCGAACCCCTGCCTCATCGCGGTGGAGCGGTATCGAACGCGCTGATCGATCGGCTTCGTGACGAGGAGGCCGTG
TGA

Structures

Toxin

• AlphaFold DB: A0A0M3F019