Detailed information of TA system TA196239

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4641748..4642548
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: A0A0K4AFE4
• Locus tag: J0O73_RS22490
• Protein ID: WP_001614005.1
• Coordinates: 4641748..4642275 (-)
• Length: 176 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: A0A8S7FBC9
• Locus tag: J0O73_RS22495
• Protein ID: WP_053883614.1
• Coordinates: 4642282..4642548 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS22465	4636823..4637590	-	768	WP_000082101.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
J0O73_RS22470	4637587..4638864	-	1278	WP_001626130.1	branched chain amino acid ABC transporter permease LivM	-
J0O73_RS22475	4638861..4639787	-	927	WP_001295111.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
J0O73_RS22480	4639835..4640944	-	1110	WP_001301528.1	high-affinity branched-chain amino acid ABC transporter substrate-binding protein LivK	-
J0O73_RS22485	4641368..4641751	+	384	WP_000778770.1	aspartate 1-decarboxylase autocleavage activator PanM	-
J0O73_RS22490	4641748..4642275	-	528	WP_001614005.1	GNAT family N-acetyltransferase	Toxin
J0O73_RS22495	4642282..4642548	-	267	WP_053883614.1	DUF1778 domain-containing protein	Antitoxin
J0O73_RS22500	4642698..4643801	-	1104	WP_001021996.1	branched chain amino acid ABC transporter substrate-binding protein LivJ	-
J0O73_RS22505	4644072..4644926	-	855	WP_000130217.1	RNA polymerase sigma factor RpoH	-
J0O73_RS22510	4645171..4646229	-	1059	WP_001042003.1	permease-like cell division protein FtsX	-
J0O73_RS22515	4646222..4646890	-	669	WP_000617723.1	cell division ATP-binding protein FtsE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (88-150)

Antitoxin

• Vibrio phage ICP1, Orf50 (8-86)

Sequences

Toxin

Protein

• Length: 176 a.a.
• Molecular weight: 19676.62 Da
• Isoelectric Point: 7.3502

>T196239 WP_001614005.1 NZ_CP071375:c4642275-4641748 [Escherichia coli]
MDDLTIEILTDDADYDLQRFDCGEEALNLFLTTHLVRQHRNKILRAYILCRNTPERQVLGYYTLSGSCFERAALPSKSKQ
KKIPYKNIPSVTLGRLAIDRSLQGQGWGATLVAHAMKVVWSASLAVGIHGLFVEALNEKAHTFYKSLGFIPLVGENENAL
FFPTKSIELLFTQSD

Nucleotide

• Length: 528 bp

>T196239 NZ_CP095023:c2184888-2184559 [Mycobacterium tuberculosis]
GTGACCGCACTTCCGGCGCGCGGAGAGGTGTGGTGGTGTGAGATGGCTGAGATCGGTCGGCGACCAGTCGTCGTGCTGTC
GCGCGATGCCGCGATCCCTCGGCTGCGACGCGCACTTGTCGCGCCCTGCACCACGACCATCCGAGGGCTAGCCAGTGAGG
TTGTTCTTGAACCCGGTTCCGACCCGATCCCGCGCCGTTCCGCGGTGAATTTGGACTCAGTCGAAAGTGTCTCGGTCGCG
GTATTGGTGAATCGGCTTGGCCGCCTCGCCGACATCCGGATGCGCGCCATCTGCACGGCCCTCGAGGTCGCCGTCGATTG
CTCTCGATGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9940.40 Da
• Isoelectric Point: 10.0863

>AT196239 WP_053883614.1 NZ_CP071375:c4642548-4642282 [Escherichia coli]
MSAVKKQRIDLRLTDDDKSMIEEAAAISNQSVSQFMLNSASQRAAEVIEQHRRVILNEESWMRVMDALSNPPSPGEKLKR
AAKRLQGM

Nucleotide

• Length: 267 bp

>AT196239 NZ_CP095023:c2185262-2184885 [Mycobacterium tuberculosis]
GTGAAGACGGCCCGGTTGCAGGTGACGTTGCGCTGCGCGGTAGACCTCATCAATTCGTCCAGCGACCAATGTTTCGCCCG
TATCGAGCACGTTGCGTCCGATCAGGCGGATCCACGCCCCGGAGTGTGGCATAGTAGTGGCATGAATCGCATACGGCTGA
GCACTACTGTCGACGCCGCACTCTTGACAAGTGCGCGCGACATGCGGGCAGGAATCACTGACGCAGCCCTCATCGATGAG
GCCCTTGCGGCATTACTGGCTCGTCATCGATCGGCCGAGGTGGATGCCAGCTATGCGGCCTACGACAAGCACCCGGTCGA
TGAGCCCGACGAGTGGGGCGATCTAGCGTCGTGGCGGCGGGCGGCTGGAGACTCGTGA

Structures

Toxin

• AlphaFold DB: A0A0K4AFE4