Detailed information of TA system TA196238

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): ataRT/DUF1778(antitoxin)
• Location: 4595703..4596457
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: ataT
• Uniprot ID: -
• Locus tag: J0O73_RS22295
• Protein ID: WP_001301452.1
• Coordinates: 4595972..4596457 (+)
• Length: 162 a.a.

Antitoxin (Protein)

• Gene name: ataR
• Uniprot ID: -
• Locus tag: J0O73_RS22290
• Protein ID: WP_000801914.1
• Coordinates: 4595703..4595981 (+)
• Length: 93 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS22285	4592931..4595636	+	2706	WP_000906960.1	HTH-type transcriptional regulator MalT	-
J0O73_RS22290	4595703..4595981	+	279	WP_000801914.1	DUF1778 domain-containing protein	Antitoxin
J0O73_RS22295	4595972..4596457	+	486	WP_001301452.1	GNAT family N-acetyltransferase	Toxin
J0O73_RS22300	4596507..4597535	-	1029	WP_053883632.1	RNA 3'-terminal phosphate cyclase	-
J0O73_RS22305	4597539..4598766	-	1228	Protein_4341	RNA-splicing ligase RtcB	-
J0O73_RS22310	4598953..4600551	+	1599	WP_153645359.1	DNA-binding transcriptional regulator RtcR	-
J0O73_RS22315	4600533..4601291	-	759	WP_000815099.1	DeoR/GlpR family transcriptional regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• GNAT domain (45-143)

Antitoxin

• Vibrio phage ICP1, Orf50 (2-92)

Sequences

Toxin

Protein

• Length: 162 a.a.
• Molecular weight: 17650.57 Da
• Isoelectric Point: 9.4066

>T196238 WP_001301452.1 NZ_CP071375:4595972-4596457 [Escherichia coli]
MGITAPTPLTSEHNLADFCCSDHGMNEWLKKKALKNHSSGLSRVYVICIANTRQVIGYYCLSTGSIQRNLAPGAMRRNAP
ESLPVVVLGRLAIDQAWAGKGLGVALLKDAVYRTMSIAQQVGVRALIVHALDDSVRNFYLKYAFVPSPFQSLTLLYPITL
E

Nucleotide

• Length: 486 bp

>T196238 NZ_CP095023:1963942-1964190 [Mycobacterium tuberculosis]
ATGGTGATCGATACCTCTGCGCTGGTCGCGATGCTCAACGATGAACCCGAGGCGCAACGGTTCGAGATAGCCGTGGCAGC
AGACCACGTTTGGCTGATGTCGACGGCGTCATATCCGGAGATGGCGACCGTGATCGAAACACGCTTCGGGGAACCGGGGG
GACGTGAACCCAAGGTCAGCGGCCAGCCTCTCCTCTATAAGGGTGACGATTTCGCATGTATCGATATTCGCGCGGTTCTC
GCCGGCTGA

Antitoxin

Protein

• Length: 93 a.a.
• Molecular weight: 10406.91 Da
• Isoelectric Point: 4.6336

>AT196238 WP_000801914.1 NZ_CP071375:4595703-4595981 [Escherichia coli]
MKPESKEAPINIRAKASQRDLIDMAANLVAKSRTDFMLDAACREAQDILLDQRLFIVDDEQYDAFLAALDAPITAERQAK
INALMNRKSPWE

Nucleotide

• Length: 279 bp

>AT196238 NZ_CP095023:1963730-1963942 [Mycobacterium tuberculosis]
ATGGAATTGGCGGCTCGAATGGGCGAGACTTTGACACAAGCGGTCGTAGTTGCAGTGCGGGAGCAACTGGCCCGCCGGAC
CGGGCGCACCAGATCCATTTCGCTACGCGAGGAGTTGGCCGCCATTGGCCGGCGCTGCGCGGCCTTACCGGTGCTCGACA
CCCGAGCCGCGGACACGATTCTCGGCTACGACGAGCGCGGGTTGCCCGCCTGA