Detailed information of TA system TA196236

Bioinformatically predicted

Overview

TA module

• Type: IV
• Classification (family/domain): cptAB/Cpta(toxin)
• Location: 4099131..4099785
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: cptA
• Uniprot ID: S1EEB2
• Locus tag: J0O73_RS19785
• Protein ID: WP_000244777.1
• Coordinates: 4099131..4099538 (-)
• Length: 136 a.a.

Antitoxin (Protein)

• Gene name: cptB
• Uniprot ID: -
• Locus tag: J0O73_RS19790
• Protein ID: WP_153645516.1
• Coordinates: 4099519..4099785 (-)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS19765	4095088..4096821	-	1734	WP_000813168.1	single-stranded-DNA-specific exonuclease RecJ	-
J0O73_RS19770	4096827..4097537	-	711	WP_000715214.1	bifunctional protein-disulfide isomerase/oxidoreductase DsbC	-
J0O73_RS19775	4097562..4098458	-	897	WP_000806638.1	site-specific tyrosine recombinase XerD	-
J0O73_RS19780	4098570..4099091	+	522	WP_001055874.1	flavodoxin FldB	-
J0O73_RS19785	4099131..4099538	-	408	WP_000244777.1	toxin CptA	Toxin
J0O73_RS19790	4099519..4099785	-	267	WP_153645516.1	FAD assembly factor SdhE	Antitoxin
J0O73_RS19795	4100028..4101008	+	981	WP_053883913.1	tRNA-modifying protein YgfZ	-
J0O73_RS19800	4101203..4101862	-	660	WP_053883911.1	hemolysin III family protein	-
J0O73_RS19805	4102026..4102337	-	312	WP_001182957.1	N(4)-acetylcytidine aminohydrolase	-
J0O73_RS19810	4102382..4103815	+	1434	WP_053883955.1	6-phospho-beta-glucosidase BglA	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Inner membrane protein YgfX (3-131)

Antitoxin

• Flavinator of succinate dehydrogenase (7-79)

Sequences

Toxin

Protein

• Length: 136 a.a.
• Molecular weight: 16031.96 Da
• Isoelectric Point: 11.5202

>T196236 WP_000244777.1 NZ_CP071375:c4099538-4099131 [Escherichia coli]
VVLWQSDLRVSWRAQWLSLLIHGLVAAVILLMPWPLSYTPLWMVLLSLVVFDCVRSQRRINARQGEIRLLMDGRLRWQGQ
EWSIVKAPWMIKSGMMLRLRSDGGKRQHLWLAADSMDEAEWRDLRRILLQQETQR

Nucleotide

• Length: 408 bp

>T196236 NZ_CP095023:1770223-1770627 [Mycobacterium tuberculosis]
ATGATCCTCATCGACACATCGGCCTGGGTGGAGTACTTCCGTGCCACCGGATCAATCGCCGCTGTCGAAGTACGCCGGCT
GCTGTCCGAAGAAGCAGCGCGAATCGCTATGTGTGAGCCCATTGCGATGGAAATCTTGAGTGGCGCGCTCGACGACAACA
CCCACACGACGCTAGAGCGGCTCGTGAATGGCTTGCCGTCGTTGAACGTTGATGACGCGATTGACTTTCGTGCTGCCGCG
GGTATCTATCGCGCCGCCCGGCGCGCCGGCGAAACGGTTCGAAGCATCAACGACTGCCTCATAGCGGCGCTCGCGATCCG
CCACGGTGCGCGTATCGTCCACCGTGACGCCGACTTTGATGTGATTGCCCGGATTACCAACCTGCAGGCCGCATCGTTTC
GGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 10517.14 Da
• Isoelectric Point: 5.6311

>AT196236 WP_153645516.1 NZ_CP071375:c4099785-4099519 [Escherichia coli]
MDINNKARIHWACRRGMRELDISIMPFFEHEYDSLSDDEKRIFIRLLECDDPDLFNWLMNHGKPADAVLEMMVRLIQTRN
RERGPVAI

Nucleotide

• Length: 267 bp

>AT196236 NZ_CP095023:1770014-1770217 [Mycobacterium tuberculosis]
ATGTCGCGCACCAACATCGACATCGATGACGAACTTGCCGCCGAGGTCATGCGCAGGTTCGGTCTGACCACCAAGAGGGC
GGCGGTCGACCTTGCCCTACGACGGTTGGTCGGGTCGCCGTTGAGCCGTGAGTTTCTGCTCGGGCTGGAAGGCGTCGGCT
GGGAAGGCGACCTGGATGACTTGCGAAGCGATCGCCCAGACTGA

Structures

Toxin

• AlphaFold DB: A0A7U9LFV7