Detailed information of TA system TA196233

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): pemIK/PRK09812-MazE
• Location: 3077270..3077860
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: pemK
• Uniprot ID: A0A8S7CSP3
• Locus tag: J0O73_RS15050
• Protein ID: WP_053884197.1
• Coordinates: 3077270..3077602 (-)
• Length: 111 a.a.

Antitoxin (Protein)

• Gene name: pemI
• Uniprot ID: A0A8S7CTL7
• Locus tag: J0O73_RS15055
• Protein ID: WP_053884198.1
• Coordinates: 3077603..3077860 (-)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS15025	3073063..3074601	-	1539	WP_053884193.1	IS66 family transposase	-
J0O73_RS15030	3074608..3074964	-	357	WP_053884194.1	IS66 family insertion sequence element accessory protein TnpB	-
J0O73_RS15035	3074951..3075262	-	312	WP_064763322.1	IS66 family insertion sequence hypothetical protein	-
J0O73_RS15040	3075536..3076420	-	885	WP_053884196.1	integrase domain-containing protein	-
J0O73_RS15045	3076558..3076859	+	302	Protein_2928	hypothetical protein	-
J0O73_RS15050	3077270..3077602	-	333	WP_053884197.1	type II toxin-antitoxin system PemK/MazF family toxin	Toxin
J0O73_RS15055	3077603..3077860	-	258	WP_053884198.1	antitoxin	Antitoxin
J0O73_RS15060	3078209..3078415	-	207	WP_053884199.1	helix-turn-helix domain-containing protein	-
J0O73_RS15065	3078412..3078873	-	462	WP_000201268.1	hypothetical protein	-
J0O73_RS15070	3079244..3080848	-	1605	WP_089553513.1	hypothetical protein	-
J0O73_RS15075	3080861..3081463	-	603	WP_207184273.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	nleB2 / nleB2	3021900..3085028	63128

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• mRNA interferase PemK-like (1-109)

Antitoxin

• Antitoxin PemI-like (4-84)

Sequences

Toxin

Protein

• Length: 111 a.a.
• Molecular weight: 11807.53 Da
• Isoelectric Point: 9.3417

>T196233 WP_053884197.1 NZ_CP071375:c3077602-3077270 [Escherichia coli]
MDRGEIWLVSLDPTTGHEQSGKRPVLIVSKASFNTFTRLPVVVPVTSGGNFAREAGFTVSLDGAGTKTTGVIRCDQPRTI
DMGARSGKRLERIPDAVVNEVLARLEAILS

Nucleotide

• Length: 333 bp

>T196233 NZ_CP095023:1541830-1542651 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9281.74 Da
• Isoelectric Point: 4.4488

>AT196233 WP_053884198.1 NZ_CP071375:c3077860-3077603 [Escherichia coli]
MYTTRLKKVGGSVMLAVPPAVLKTLGLSTDSEVGMTIDDGCLIIEPQKRPRYSLEELLAQCDPHAEISEEGREWIDAPAV
GKEIL

Nucleotide

• Length: 258 bp

>AT196233 NZ_CP095023:1542644-1542880 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG