Detailed information of TA system TA196218

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): paaR-paaA-parE/-
• Location: 1877094..1877572
• Replicon: chromosome
• Accession: NZ_CP071375
• Organism: Escherichia coli strain E105

Toxin (Protein)

• Gene name: parE_1
• Uniprot ID: A0A891SFN9
• Locus tag: J0O73_RS08825
• Protein ID: WP_001303876.1
• Coordinates: 1877094..1877381 (-)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: paaA
• Uniprot ID: Q8XD67
• Locus tag: J0O73_RS08830
• Protein ID: WP_000536233.1
• Coordinates: 1877381..1877572 (-)
• Length: 64 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0O73_RS08795	1873496..1873687	-	192	WP_001098307.1	DUF1482 family protein	-
J0O73_RS08800	1873684..1873872	-	189	WP_000413705.1	cell division inhibition protein DicB	-
J0O73_RS08805	1874446..1874631	+	186	WP_001133046.1	hypothetical protein	-
J0O73_RS08810	1874818..1875207	-	390	WP_153645614.1	hypothetical protein	-
J0O73_RS08820	1876662..1876817	-	156	WP_000379575.1	DUF1391 family protein	-
J0O73_RS08825	1877094..1877381	-	288	WP_001303876.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
J0O73_RS08830	1877381..1877572	-	192	WP_000536233.1	hypothetical protein	Antitoxin
J0O73_RS08835	1877600..1878001	-	402	WP_153645675.1	helix-turn-helix domain-containing protein	-
J0O73_RS08840	1878110..1878382	+	273	WP_000887453.1	helix-turn-helix domain-containing protein	-
J0O73_RS08845	1878366..1878791	+	426	WP_000693855.1	Rha family transcriptional regulator	-
J0O73_RS08850	1878998..1879453	-	456	WP_000273724.1	hypothetical protein	-
J0O73_RS08855	1879532..1880641	+	1110	WP_153645676.1	DNA-binding protein	-
J0O73_RS08860	1880648..1881334	+	687	WP_000788749.1	ATP-binding protein	-
J0O73_RS08865	1881416..1882186	+	771	WP_000450992.1	DUF1627 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Integrative and Conjugative Element	-	-	1862269..1886687	24418

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (4-91)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 10978.84 Da
• Isoelectric Point: 10.1360

>T196218 WP_001303876.1 NZ_CP071375:c1877381-1877094 [Escherichia coli]
MLPILWLPSARDDLRQIITYIAKENPPAARRLKIRIETSVLPLSEHPYLYPPSERVSGLREIVTHPNYIILYRVAASSIE
IVSVTHSRRQFPFSI

Nucleotide

• Length: 288 bp

>T196218 NZ_CP095023:c698882-698469 [Mycobacterium tuberculosis]
GTGAAACCGCCGCTGGCAGTGGACACCAGCGTCGCGATTCCGTTGCTGGTACGGACCCATACCGCGCATGCGGCGGTTGT
CGCCTGGTGGGCACATCGGGAGGCCGCGCTGTGCGGGCACGCCCTGGCCGAAACCTACTCCGTGCTGACCCGCCTGCCGC
GTGACCTACGACTGGCACCGATGGACGCCGCGCGGCTTCTTACCGAACGCTTTGCGGCACCGCTGTTGTTGAGTTCCCGC
ACCACTGAACATCTCCCACGCGTGCTGGCCCAATTCGAGATCACTGGTGGCGCGGTCTATGACGCTCTCGTGGCCCTTGC
CGCCGCCGAGCACCGGGCTGAACTCGCCACACGCGATGCTCGAGCCAAGGACACTTACGAAAAGATCGGCGTCCACGTCG
TTGTCGCCGCGTGA

Antitoxin

Protein

• Length: 64 a.a.
• Molecular weight: 7025.80 Da
• Isoelectric Point: 4.0270

>AT196218 WP_000536233.1 NZ_CP071375:c1877572-1877381 [Escherichia coli]
MGTALSPIVSEFETTEQENSYNEWLRTKVTSSLADTRPAIPHDEVMAEMENLIAQIAVTNKSE

Nucleotide

• Length: 192 bp

>AT196218 NZ_CP095023:c699115-698879 [Mycobacterium tuberculosis]
ATGAAGGCTGTTGTGGATGCGGCTGGCCGCATCGTCGTACCCAAACCGCTGCGCGAAGCCTTGGGGCTGCAACCGGGGTC
CACCGTGGAAATATCCCGCTATGGCGCTGGTCTCCACCTGATTCCCACCGGACGAACAGCGCGTCTCGAAGAGGAGAATG
GCGTACTGGTGGCCACCGGTGAGACCACGATCGACGATGAGGTCGTGTTCGGCCTTATCGATAGCGGCCGGAAGTGA