Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) vapBC/LOTUS_5_Limkain_b1(toxin)
Location 2995237..2995862 Replicon chromosome
Accession NZ_CP071324
Organism Klebsiella pneumoniae strain JS161205112

Toxin (Protein)


Gene name vapC Uniprot ID R4Y4A3
Locus tag J0G22_RS15520 Protein ID WP_002882817.1
Coordinates 2995479..2995862 (+) Length 128 a.a.

Antitoxin (Protein)


Gene name vapB Uniprot ID J2DFR0
Locus tag J0G22_RS15515 Protein ID WP_004150355.1
Coordinates 2995237..2995479 (+) Length 81 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
J0G22_RS15490 2991228..2991827 + 600 WP_004901669.1 glucose-1-phosphatase -
J0G22_RS15495 2991821..2992681 + 861 WP_002882807.1 virulence factor BrkB family protein -
J0G22_RS15500 2992678..2993115 + 438 WP_002882809.1 D-tyrosyl-tRNA(Tyr) deacylase -
J0G22_RS15505 2993160..2994101 + 942 WP_004178031.1 fatty acid biosynthesis protein FabY -
J0G22_RS15510 2994115..2995032 - 918 WP_029884108.1 alpha/beta hydrolase -
J0G22_RS15515 2995237..2995479 + 243 WP_004150355.1 ribbon-helix-helix domain-containing protein Antitoxin
J0G22_RS15520 2995479..2995862 + 384 WP_002882817.1 type II toxin-antitoxin system VapC family toxin Toxin
J0G22_RS15525 2996036..2996965 - 930 WP_004150358.1 formate dehydrogenase accessory protein FdhE -
J0G22_RS15530 2996962..2997597 - 636 WP_206921240.1 formate dehydrogenase cytochrome b556 subunit -
J0G22_RS15535 2997594..2998496 - 903 WP_002882822.1 formate dehydrogenase subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

PIN domain

(7-112)

Antitoxin

Predicted DNA-binding protein, ribbon-helix-helix domain

(16-47)


Sequences

Toxin        


Download         Length: 128 a.a.        Molecular weight: 14377.62 Da        Isoelectric Point: 7.3178

>T196209 WP_002882817.1 NZ_CP071324:2995479-2995862 [Klebsiella pneumoniae]
MTSGSALFDTNILIDLFSGRREAKQALEAWPPQNAISLITWMEVMVGAKKYHQEQRTRMALSTFNIINISQDIAERSVAL
RQEYKLKLPDAIILATAQLHRLELITRNTKDFAGIPGVVTPYEIHPE

Download         Length: 384 bp

>T196209 NZ_CP095022:3831399-3831809 [Mycobacterium tuberculosis]
GTGATCTATATGGACACCTCGGCCCTGACTAAGCTGCTCATCTCCGAGCCCGAGACGACCGAACTGCGGACATGGCTGAC
CGCGCAAAGCGGCCAGGGCGAGGACGCGGCGACAAGCACCCTTGGCCGGGTCGAGTTGATGAGAGTCGTTGCCCGATACG
GACAACCAGGCCAAACTGAGCGTGCGCGTTACCTACTCGACGGGCTCGACATCCTCCCGCTCACCGAACCGGTGATCGGT
CTAGCTGAAACGATCGGACCGGCCACCCTACGTTCTCTCGACGCGATTCACCTCGCGGCCGCAGCCCAGATCAAGCGGGA
ACTGACAGCCTTCGTCACCTACGACCACCGATTGTTGAGCGGATGCCGTGAGGTCGGCTTCGTCACCGCCTCACCCGGCG
CAGTCCGGTGA

Antitoxin


Download         Length: 81 a.a.        Molecular weight: 9567.83 Da        Isoelectric Point: 4.3509

>AT196209 WP_004150355.1 NZ_CP071324:2995237-2995479 [Klebsiella pneumoniae]
MMAERDMGRILLDLSDDVIQRLDDLKVQRNIPRAELLREAVEQYLEKQDRAKDTISSALGLWQDCEEDGMEYQRQLRKEW

Download         Length: 243 bp

>AT196209 NZ_CP095022:3831124-3831402 [Mycobacterium tuberculosis]
GTGGAGGCAATCGGAATCCGAGAACTAAGACAGCACGCATCGCGATACCTCGCCCGGGTTGAAGCCGGCGAGGAACTTGG
CGTCACCAACAAAGGAAGACTTGTGGCCCGACTCATCCCGGTGCAGGCCGCGGAGCGTTCTCGCGAAGCCCTGATTGAAT
CAGGTGTCCTGATTCCGGCTCGTCGTCCACAAAACCTTCTCGACGTCACCGCCGAACCGGCGCGCGGCTGCAAGCGCACC
CTGTCCGATGTTCTCAACGAAATGCGCGACGAGCAGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0H3GPK8

Antitoxin

Source ID Structure
AlphaFold DB A0A0H3GGU9

References