Detailed information of TA system TA196196

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): hipBA/HipA-HipB
• Location: 66421..68009
• Replicon: chromosome
• Accession: NZ_CP071324
• Organism: Klebsiella pneumoniae strain JS161205112

Toxin (Protein)

• Gene name: hipA
• Uniprot ID: W8UI36
• Locus tag: J0G22_RS01385
• Protein ID: WP_002904792.1
• Coordinates: 66687..68009 (+)
• Length: 441 a.a.

Antitoxin (Protein)

• Gene name: hipB
• Uniprot ID: -
• Locus tag: J0G22_RS01380
• Protein ID: WP_004176198.1
• Coordinates: 66421..66687 (+)
• Length: 89 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G22_RS01355	61450..62373	+	924	WP_002904781.1	LysR family hpxDE operon transcriptional regulator HpxR	-
J0G22_RS01360	62354..63508	-	1155	WP_002904783.1	FAD-dependent urate hydroxylase HpxO	-
J0G22_RS01365	63854..65233	+	1380	WP_014599164.1	purine permease	-
J0G22_RS01370	65211..65711	+	501	WP_004224756.1	2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline decarboxylase	-
J0G22_RS01375	65708..66034	+	327	WP_004224757.1	hydroxyisourate hydrolase	-
J0G22_RS01380	66421..66687	+	267	WP_004176198.1	type II toxin-antitoxin system antitoxin HipB	Antitoxin
J0G22_RS01385	66687..68009	+	1323	WP_002904792.1	type II toxin-antitoxin system HipA family toxin	Toxin
J0G22_RS01390	68026..69318	-	1293	WP_016946381.1	glycoside hydrolase family 10 protein	-
J0G22_RS01395	69460..70464	-	1005	WP_029884185.1	type 1 fimbrial protein	-
J0G22_RS01400	70452..72953	-	2502	WP_029884184.1	fimbrial biogenesis outer membrane usher protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• HipA-like, C-terminal (149-402)

Antitoxin

• Cro/C1-type helix-turn-helix domain (13-65)

Sequences

Toxin

Protein

• Length: 441 a.a.
• Molecular weight: 49171.46 Da
• Isoelectric Point: 7.5357

>T196196 WP_002904792.1 NZ_CP071324:66687-68009 [Klebsiella pneumoniae]
MATLTTWMNNVRVGTLTRQANGAHSFRYDEEWLRSPRARPLSLSLPLQYGNITADAVYHYFDNLLPDSPQVRDRIVRRYQ
ARSKQPFDLLAEVGRDSVGAVTLLPPGEEAHLEGLRWQTLDEAQLTALLTAYQSDIPLGMITSQDDFRISVAGAQEKTAL
LRMGEQWCIPQGATPTTHIIKLPIGEIKQPNATLDLRESVDNEYLCLALARELGLAVPEAEIITTPRIRALAVTRFDRRW
AQEGRVLLRLPQEDLCQAFGLPSAMKYESDGGPGIAAIMTFLLGSSEALKDRYDFMKFMVFQWLTGATDGHAKNFSIYLL
PGGSYRLTPFYDIISAFPVLGGTGLHLRDLKLSMGLNATKGRKTEINAIYPRHFLATAKAVNFPREQMLAILQEFAGHVP
QAIESARRTLPADFSSHVWQAITENMLKLHARLQQGLQAL

Nucleotide

• Length: 1323 bp

>T196196 NZ_CP095022:2927409-2927849 [Mycobacterium tuberculosis]
ATGCTGCTCTGTGACACCAACATCTGGCTGGCGTTGGCGCTTTCCGGACACGTGCACCACAGGGCCTCGCGCGCATGGCT
AGACACCATCAACGCGCCCGGAGTCATCCACTTTTGCCGCGCAACCCAACAGTCGCTCCTTCGGCTGTTGACGAATCGGA
CGGTGCTGGGCGCGTATGGCAGCCCACCACTGACCAACCGCGAAGCGTGGGCGGCCTATGCCGCGTTCCTGGATGACGAC
CGCATCGTGCTGGCCGGCGCCGAACCTGATGGTTTGGAGGCCCAGTGGAGAGCCTTCGCCGTTCGCCAGTCGCCGGCGCC
CAAGGTTTGGATGGATGCCTACCTAGCTGCTTTCGCACTTACCGGTGGATTCGAGTTGGTGACGACTGACACCGCCTTCA
CCCAGTACGGCGGAATCGAGCTGCGGCTCCTGGCCAAGTGA

Antitoxin

Protein

• Length: 89 a.a.
• Molecular weight: 9936.36 Da
• Isoelectric Point: 8.5912

>AT196196 WP_004176198.1 NZ_CP071324:66421-66687 [Klebsiella pneumoniae]
MNSPQIYSPVQLANYLKLLRQQNHWTQDALARRIGIKQATLSNFENHPDKTTLTTLFKILQSLGVVMMLNAKAAETAPAP
DNNTDLDW

Nucleotide

• Length: 267 bp

>AT196196 NZ_CP095022:2927135-2927422 [Mycobacterium tuberculosis]
ATGAAGACCACGCTCGACCTGCCTGATGAACTGATGCGCGCTATCAAGGTCCGCGCGGCGCAGCAGGGCCGCAAGATGAA
AGATGTCGTGACCGAACTGCTCAGATCCGGTCTGTCCCAGACGCACAGCGGGGCTCCAATCCCAACGCCGCGGCGCGTGC
AGCTTCCCCTGGTGCATTGCGGTGGCGCGGCTACCCGCGAACAAGAAATGACGCCGGAGCGTGTTGCCGCGGCCTTGCTC
GACCAGGAGGCCCAGTGGTGGTCCGGACACGACGATGCTGCTCTGTGA

Structures

Toxin

• AlphaFold DB: A0A0E1CEC8