Detailed information of TA system TA196194

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vagCD/VapC-VagC
• Location: 21103..21773
• Replicon: plasmid pVP-JS4
• Accession: NZ_CP071323
• Organism: Klebsiella pneumoniae strain JS161205112

Toxin (Protein)

• Gene name: vagD
• Uniprot ID: Q6U619
• Locus tag: J0G22_RS00125
• Protein ID: WP_004213072.1
• Coordinates: 21103..21546 (-)
• Length: 148 a.a.

Antitoxin (Protein)

• Gene name: vagC
• Uniprot ID: Q6U620
• Locus tag: J0G22_RS00130
• Protein ID: WP_004213073.1
• Coordinates: 21543..21773 (-)
• Length: 77 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0G22_RS00090	16510..16785	+	276	WP_004213932.1	carboxymuconolactone decarboxylase family protein	-
J0G22_RS00095	16848..17339	+	492	WP_011251327.1	DM13 domain-containing protein	-
J0G22_RS00100	17388..18308	+	921	WP_029884592.1	DUF1471 domain-containing protein	-
J0G22_RS00105	18399..18805	+	407	Protein_20	GAF domain-containing protein	-
J0G22_RS00110	19321..19959	-	639	Protein_21	response regulator	-
J0G22_RS00115	20376..20669	+	294	Protein_22	transposase	-
J0G22_RS00120	20673..21089	+	417	Protein_23	restriction endonuclease	-
J0G22_RS00125	21103..21546	-	444	WP_004213072.1	type II toxin-antitoxin system VapC family toxin	Toxin
J0G22_RS00130	21543..21773	-	231	WP_004213073.1	AbrB/MazE/SpoVT family DNA-binding domain-containing protein	Antitoxin
J0G22_RS00135	22381..23514	+	1134	WP_004213075.1	DUF3800 domain-containing protein	-
J0G22_RS00140	23530..23823	+	294	WP_004213076.1	hypothetical protein	-
J0G22_RS00145	23813..24019	-	207	WP_004213077.1	hypothetical protein	-
J0G22_RS00150	24371..24661	+	291	WP_004213078.1	hypothetical protein	-
J0G22_RS00155	24651..25550	+	900	WP_004225022.1	nucleotide-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Mobilizable plasmid	-	iucA / iucB / iucC / iucD / iutA / rmpA2 / rmpA / iroN / iroD / iroC / iroB	1..180641	180641

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (6-129)

Antitoxin

• SpoVT-AbrB domain (7-47)

Sequences

Toxin

Protein

• Length: 148 a.a.
• Molecular weight: 16006.74 Da
• Isoelectric Point: 9.3642

>T196194 WP_004213072.1 NZ_CP071323:c21546-21103 [Klebsiella pneumoniae]
MKKTWMLDTNICSFIMREQPAAVLKRLEQAVLRGDRIVVSAVTYAEMRFGATGPKASPRHIQLVDAFCARLDAILPWDRA
AVDATTDIRVALRLAGTPIGPNDTAIAGHAIAAGAVLVTNNVREFERVPGLVLEDWVKKAALCRVIS

Nucleotide

• Length: 444 bp

>T196194 NZ_CP095022:2865927-2866304 [Mycobacterium tuberculosis]
GTGAAGCTGATCGACACCACCATCGCGGTCGACCACCTTCGCGGCGAACCCGCGGCAGCCGTGCTGCTCGCCGAACTGAT
AAACAACGGTGAGGAGATCGCGGCCAGCGAGCTGGTCCGATTCGAACTCCTCGCCGGTGTGCGGGAAAGCGAACTCGCGG
CGCTCGAGGCCTTCTTCTCGGCAGTGGTGTGGACCCTGGTGACCGAGGACATTGCCCGGATCGGCGGACGACTCGCCCGT
CGATACCGGTCCAGCCACCGCGGTATCGACGACGTGGACTACCTGATCGCTGCGACCGCCATTGTGGTCGACGCCGACCT
GCTCACCACCAATGTGCGCCACTTCCCGATGTTCCCGGATCTGCAGCCGCCGTACTGA

Antitoxin

Protein

• Length: 77 a.a.
• Molecular weight: 8677.73 Da
• Isoelectric Point: 4.4563

>AT196194 WP_004213073.1 NZ_CP071323:c21773-21543 [Klebsiella pneumoniae]
MRTVSIFKNGNNRAIRLPRDLDFDGVSELEIVREGDSIILRPVRPTWGSFAQLDRAAPDFMAEREDVVSDEGRFEP

Nucleotide

• Length: 231 bp

>AT196194 NZ_CP095022:2865673-2865930 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Toxin

• AlphaFold DB: Q6U619

Antitoxin

• AlphaFold DB: Q6U620