Detailed information of TA system TA196193

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): vapBC/LOTUS_5_Limkain_b1(toxin)
• Location: 5022476..5023077
• Replicon: chromosome
• Accession: NZ_CP071320
• Organism: Serratia ureilytica strain T6

Toxin (Protein)

• Gene name: vapC
• Uniprot ID: -
• Locus tag: J0X03_RS23750
• Protein ID: WP_160203877.1
• Coordinates: 5022697..5023077 (+)
• Length: 127 a.a.

Antitoxin (Protein)

• Gene name: vapB
• Uniprot ID: A0A8B4GSF3
• Locus tag: J0X03_RS23745
• Protein ID: WP_025304767.1
• Coordinates: 5022476..5022697 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0X03_RS23730	5018126..5019265	-	1140	WP_046687995.1	PLP-dependent aspartate aminotransferase family protein	-
J0X03_RS23735	5019345..5020124	-	780	WP_033645137.1	2OG-Fe dioxygenase family protein	-
J0X03_RS23740	5021152..5022402	+	1251	WP_033645138.1	valine--pyruvate transaminase	-
J0X03_RS23745	5022476..5022697	+	222	WP_025304767.1	ribbon-helix-helix domain-containing protein	Antitoxin
J0X03_RS23750	5022697..5023077	+	381	WP_160203877.1	type II toxin-antitoxin system VapC family toxin	Toxin
J0X03_RS23755	5023088..5024746	-	1659	WP_042783386.1	putative transporter	-
J0X03_RS23760	5024914..5025342	-	429	WP_004933922.1	heat shock chaperone IbpB	-
J0X03_RS23765	5025449..5025862	-	414	WP_004933919.1	heat shock chaperone IbpA	-
J0X03_RS23770	5026172..5026519	+	348	WP_016929593.1	YceK/YidQ family lipoprotein	-
J0X03_RS23775	5026550..5027830	-	1281	WP_103101713.1	DUF3748 domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• PIN domain (7-100)

Antitoxin

• Ribbon-helix-helix protein, CopG (3-40)

Sequences

Toxin

Protein

• Length: 127 a.a.
• Molecular weight: 14226.69 Da
• Isoelectric Point: 7.3173

>T196193 WP_160203877.1 NZ_CP071320:5022697-5023077 [Serratia ureilytica]
MVAQRALFDTNILIDYLNGIPQARDVLVEYHINPAISAITWMEVMVGAKKQGPALELKTRLFLGQFLLLPITDEVAERAV
ELRHSQHVKLPDAIIWATAQVGFRMLISRNPKDFGTDNGVLMPYRL

Nucleotide

• Length: 381 bp

>T196193 NZ_CP095022:2865221-2865634 [Mycobacterium tuberculosis]
ATGGTGTTCTGCGTCGACACCAGCGCGTGGCATCACGCGGCGCGGCCGGAAGTTGCGCGCCGATGGTTGGCGGCCTTGTC
CGCGGACCAGATCGGCATCTGCGACCACGTGCGGTTGGAGATCCTGTACTCGGCGAACTCCGCTACCGACTACGACGCGC
TCGCCGACGAACTCGACGGCTTGGCCCGTATACCAGTCGGTGCCGAAACCTTTACGCGCGCATGCCAAGTCCAGCGTGAG
CTTGCCCACGTCGCCGGTCTGCATCACCGCAGCGTGAAGATCGCCGATCTTGTCATCGCCGCGGCGGCCGAACTTTCAGG
CACCATCGTGTGGCATTACGACGAGAACTATGACCGGGTCGCCGCCATCACCGGCCAACCTACGGAGTGGATCGTGCCGC
GCGGGACCCTTTAA

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8607.55 Da
• Isoelectric Point: 4.4915

>AT196193 WP_025304767.1 NZ_CP071320:5022476-5022697 [Serratia ureilytica]
MTRIVIDLPEEDLRQLDNLKNIRHVPRAEIIRQAVSRYLTENHVEDPSNAFGLWSGAMGDGVEYENRQREEWE

Nucleotide

• Length: 222 bp

>AT196193 NZ_CP095022:2865673-2865930 [Mycobacterium tuberculosis]
ATGCGGACTCAGGTGACCCTGGGCAAAGAGGAGCTTGAGCTGCTCGATCGTGCCGCCAAGGCGAGTGGCGCATCGCGGTC
CGAACTCATCCGACGCGCAATTCACCGTGCCTACGGGACTGGATCCAAGCAGGAACGGCTCGCCGCGCTCGACCACAGCC
GTGGCTCGTGGCGAGGACGGGACTTCACCGGCACCGAGTATGTCGACGCCATTCGGGGCGACCTCAACGAACGACTTGCT
CGGCTCGGTCTGGCGTGA

Structures

Antitoxin

• AlphaFold DB: A0A8B4GSF3