Detailed information of TA system TA196175

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): BrnTA/BrnT-BrnA
• Location: 2746333..2746948
• Replicon: chromosome
• Accession: NZ_CP071319
• Organism: Acinetobacter indicus strain GXNN62X4

Toxin (Protein)

• Gene name: brnT
• Uniprot ID: N9P1T1
• Locus tag: J0W33_RS13115
• Protein ID: WP_005244631.1
• Coordinates: 2746333..2746620 (+)
• Length: 96 a.a.

Antitoxin (Protein)

• Gene name: brnA
• Uniprot ID: -
• Locus tag: J0W33_RS13120
• Protein ID: WP_206896762.1
• Coordinates: 2746607..2746948 (+)
• Length: 114 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
J0W33_RS13090	2741346..2742734	-	1389	WP_005176761.1	adenylosuccinate lyase	-
J0W33_RS13095	2742785..2743516	-	732	WP_206896760.1	high frequency lysogenization protein HflD	-
J0W33_RS13100	2743531..2744664	-	1134	WP_075166642.1	tRNA 2-thiouridine(34) synthase MnmA	-
J0W33_RS13105	2744669..2745154	-	486	WP_005176771.1	NUDIX hydrolase	-
J0W33_RS13110	2745444..2746052	+	609	WP_206896761.1	hypothetical protein	-
J0W33_RS13115	2746333..2746620	+	288	WP_005244631.1	BrnT family toxin	Toxin
J0W33_RS13120	2746607..2746948	+	342	WP_206896762.1	BrnA antitoxin family protein	Antitoxin
J0W33_RS13125	2746986..2747402	-	417	WP_005182334.1	hypothetical protein	-
J0W33_RS13130	2747593..2748123	-	531	WP_016658409.1	gamma carbonic anhydrase family protein	-
J0W33_RS13135	2748230..2749378	-	1149	WP_177155494.1	D-alanyl-D-alanine carboxypeptidase PBP5/6	-
J0W33_RS13140	2749496..2749891	-	396	WP_016658407.1	hypothetical protein	-
J0W33_RS13145	2750089..2750460	-	372	WP_005176804.1	hypothetical protein	-
J0W33_RS13150	2750571..2751347	+	777	WP_075174788.1	5'/3'-nucleotidase SurE	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Ribonuclease toxin, BrnT, of type II toxin-antitoxin system (7-88)

Antitoxin

• BrnA antitoxin of type II toxin-antitoxin system (41-96)

Sequences

Toxin

Protein

• Length: 96 a.a.
• Molecular weight: 11291.66 Da
• Isoelectric Point: 5.2134

>T196175 WP_005244631.1 NZ_CP071319:2746333-2746620 [Acinetobacter indicus]
MEQYFEWDEAKNRKNQKKHDISFETASLVFDDPLRISIQDRHTDGEERWQTIGKVKGVLMLLVAHTIFDEDDCEIIRIIS
ARQVTKAERDRYEHG

Nucleotide

• Length: 288 bp

>T196175 NZ_CP095022:1539880-1540701 [Mycobacterium tuberculosis]
GTGGTGGTAGCCCTCGTAGGCTCAGCAATCGTGGACTTGCATTCACGACCACCGTGGTCGAACAACGCGGTGCGTCGTCT
TGGCGTGGCACTGCGCGACGGAGTTGACCCGCCGGTCGACTGCCCGTCGTACGCCGAGGTGATGCTGTGGCATGCGGACT
TGGCCGCCGAAGTCCAGGACCGGATCGAGGGCCGGAGTTGGTCTGCGTCGGAGTTATTGGTTACCTCACGTGCGAAGAGC
CAAGACACCCTGCTAGCAAAGCTGCGGCGTCGGCCTTACCTGCAACTGAACACCATCCAAGACATCGCAGGTGTCCGCAT
CGATGCCGACCTCCTGCTGGGCGAGCAGACGAGACTTGCTCGCGAGATCGCCGACCACTTCGGTGCTGACCAGCCCGCTA
TTCATGATCTGCGTGACCACCCGCACGCCGGCTACCGGGCCGTTCATGTCTGGCTTCGGTTACCTGCCGGTCGTGTCGAG
ATACAGATTCGCACCATTTTGCAGAGCCTGTGGGCCAACTTCTACGAGCTTCTCGCTGACGCGTACGGTCGGGGCATCCG
CTATGACGAGCGGCCGGAGCAGCTAGCGGCCGGCGTTGTCCCGGCACAGCTTCAAGAGCTGGTAGGGGTTATGCAAGACG
CTTCAGCGGATCTGGCGATGCATGAAGCCGAGTGGCAACACTGTGCAGAGATCGAATACCCCGGCCAGCGGGCGATGGCG
CTTGGCGAGGCGAGCAAGAACAAGGCGACGGTGCTCGCAACGACCAAGTTTAGGCTGGAAAGGGCCATCAATGAGGCCGA
GTCGGCAGGGGGAGGTGGGTGA

Antitoxin

Protein

• Length: 114 a.a.
• Molecular weight: 13172.97 Da
• Isoelectric Point: 8.4749

>AT196175 WP_206896762.1 NZ_CP071319:2746607-2746948 [Acinetobacter indicus]
MSMVRYSRKELNEKFSDKQDAEIERLLAKGTVPDDQLDLSDIPEITDWSNAVRHNQFYRPVKQQTSIRLDADVLAWFKAQ
GKGYQTRMNEILRDAMLKELKIINKNGSLSSHF

Nucleotide

• Length: 342 bp

>AT196175 NZ_CP095022:1540694-1540930 [Mycobacterium tuberculosis]
GTGGGTGAGGTGGCTGGCTATGTCGTCGAATACAACCGGCGCACCCACGTGCGTCGCATCACCGAGTTCGCCACCCCGCA
AGAAGCGATGGAGCACCGGTTGAAGCTGGAAGCCGAGCGCACCGACAGCAATATCGAGATCGTTGCGCTCGTCAGTAAGT
CGTTGGGAACCCTGAAGCAAACGCATTCGCGGTACTTCACTGGTGAAGAGCTGAACGTCGGAAACGGCGCGCGGTAG

Structures

Toxin

• AlphaFold DB: N9P1T1